The role of satellite cell-derived TRIM28 in mechanical load- and injury-induced myogenesis.

Autor: Lin KH; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA., Hibbert JE; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA., Lemens JL; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA., Torbey MM; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA., Steinert ND; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA., Flejsierowicz PM; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA., Melka KM; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA., Lares M; Department of Dermatology, University of Wisconsin - Madison, WI, USA., Setaluri V; Department of Dermatology, University of Wisconsin - Madison, WI, USA., Hornberger TA; Department of Comparative Biosciences, University of Wisconsin - Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, WI, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2023 Dec 29. Date of Electronic Publication: 2023 Dec 29.
DOI: 10.1101/2023.12.20.572566
Abstrakt: Satellite cells are skeletal muscle stem cells that contribute to postnatal muscle growth, and they endow skeletal muscle with the ability to regenerate after a severe injury. Here we discovered that this myogenic potential of satellite cells requires a protein called tripartite motif-containing 28 (TRIM28). Unexpectedly, multiple lines of both in vitro and in vivo evidence revealed that the myogenic function of TRIM28 is not dependent on changes in the phosphorylation of its serine 473 residue. Moreover, the functions of TRIM28 were not mediated through the regulation of satellite cell proliferation or differentiation. Instead, our findings indicate that TRIM28 regulates the ability of satellite cells to progress through the process of fusion. Specifically, we discovered that TRIM28 controls the expression of a fusogenic protein called myomixer and concomitant fusion pore formation. Collectively, the outcomes of this study expose the framework of a novel regulatory pathway that is essential for myogenesis.
Databáze: MEDLINE