RNA-seq unravels distinct expression profiles of keloids and Dupuytren's disease.

Autor:	Stocks M; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany., Walter AS; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany., Akova E; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany., Gauglitz G; Department of Dermatology and Allergy, University Hospital, LMU, Thalkirchnerstr. 48, 80337 Munich, Germany., Aszodi A; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany., Boecker W; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany., Saller MM; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany., Volkmer E; Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), University Hospital, Ludwig-Maximillians-University (LMU), Frauenhoferstr. 12, 80336 Munich, Germany.; Clinic of Hand Surgery, Helios Klinikum Muenchen West, Steinerweg 5, 81241 Munich, Germany.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Dec 13; Vol. 10 (1), pp. e23681. Date of Electronic Publication: 2023 Dec 13 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2023.e23681
Abstrakt:	Keloid scars and Dupuytren's disease are two common, chronic, and incurable fibroproliferative disorders that, among other shared clinical features, may induce joint contractures. We employed bulk RNA sequencing to discern potential shared gene expression patterns and underlying pathological pathways between these two conditions. Our aim was to uncover potential molecular targets that could pave the way for novel therapeutic strategies. Differentially expressed genes (DEGs) were functionally annotated using Gene Ontology (GO) terms and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways with the Database for Annotation, Visualization, and Integrated Discovery (DAVID). The protein-protein-interaction (PPI) networks were constructed by using the Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape. The Molecular Complex Detection (MCODE) plugin was used for downstream analysis of the PPI networks. A total of 1922 DEGs were identified within Dupuytren's and keloid samples, yet no overlapping gene expression profiles were detected. Significantly enriched GO terms were related to skin development and tendon formation in keloid scars and Dupuytren's disease, respectively. The PPI network analysis revealed 10 genes and the module analysis provided six protein networks, which might play an integral part in disease development. These genes, including CDH1, ERBB2, CASP3 and RPS27A, may serve as new targets for future research to develop biomarkers and/or therapeutic agents. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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