Urinary matrix metalloproteinase-7 is a sensitive biomarker to evaluate renal tubular injury in patients with minimal change disease and focal segmental glomerulosclerosis.

Autor: Yin DY; Department of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, China., Hou GL; Department of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, China., Yang XQ; Department of Pediatrics, The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China., Bi LL; Department of Pediatrics, The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China., Mei XF; Department of Pediatrics, The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China., Bai MK; Department of Pediatrics, The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China., Zhou L; School of Pharmacy China Pharmaceutical University, Nanjing, Jiangsu, China., Zhu S; Department of Pediatrics, Henan Province Hospital of TCM, Zhengzhou, Henan, China., Huang YJ; Department of Pediatrics, Henan University of Chinese Medicine, Zhengzhou, Henan, China.; Department of Pediatrics, The First Affiliated Hospital of Henan University of CM, Zhengzhou, Henan, China.
Jazyk: angličtina
Zdroj: Clinical kidney journal [Clin Kidney J] 2023 Feb 10; Vol. 17 (1), pp. sfad027. Date of Electronic Publication: 2023 Feb 10 (Print Publication: 2024).
DOI: 10.1093/ckj/sfad027
Abstrakt: Objective: To explore the advantages of urinary matrix metalloproteinase-7 (MMP-7) in evaluating renal tubular injury in minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) patients compared with urinary cystatin C (CysC) and retinol-binding protein (RBP).
Methods: Serum and urine samples were collected from 20 healthy volunteers, and 40 MCD and 20 FSGS patients. Serum and urinary MMP-7 levels were measured by enzyme-linked immunosorbent assay. Urinary total protein, CysC and RBP levels were measured by automatic specific protein analyzer and compared with urinary creatinine level for calibration. The renal tissue serial sections were stained by MMP-7 immunohistochemistry and periodic acid-Schiff.
Results: Under light microscopy, MMP-7 granular weak positive expression was showed sporadically in the cytoplasm of a few renal tubular epithelial cells without obvious morphological changes in MCD patients, and MMP-7-positive expression was observed in the cytoplasm of some renal tubular epithelial cells in FSGS patients. There was no significant difference in serum MMP-7 level among the three groups. Compared with the control group, the urinary MMP-7 level in MCD patients was higher, but urinary CysC and RBP levels were not increased significantly. Compared with the control group and MCD patients, urinary MMP-7, CysC and RBP levels in FSGS patients were upregulated significantly.
Conclusions: Urinary MMP-7 could not only evaluate the mild renal tubular epithelial cells injury in MCD patients with massive proteinuria, but also evaluate the continuous renal tubular epithelial cells injury in FSGS patients.
Competing Interests: No conflicts of interest (financial or otherwise) are declared by the authors.
(© The Author(s) 2023. Published by Oxford University Press on behalf of the ERA.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje