Human organotypic brain slice cultures: a detailed and improved protocol for preparation and long-term maintenance.

Autor: Bak A; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany. Electronic address: aniella.va.bak@gmail.com., Koch H; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., van Loo KMJ; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany., Schmied K; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., Gittel B; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., Weber Y; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., Ort J; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany., Schwarz N; Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany., Tauber SC; Department of Neurology, RWTH Aachen University Hospital, Aachen, Germany., Wuttke TV; Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; Department of Neurosurgery, University of Tübingen, Tübingen, Germany., Delev D; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany; Neurosurgical Artificial Intelligence Laboratory Aachen (NAILA), RWTH Aachen University Hospital, Aachen, Germany; Department of Neurosurgery, University of Erlangen, Erlangen, Germany.
Jazyk: angličtina
Zdroj: Journal of neuroscience methods [J Neurosci Methods] 2024 Apr; Vol. 404, pp. 110055. Date of Electronic Publication: 2024 Jan 05.
DOI: 10.1016/j.jneumeth.2023.110055
Abstrakt: The investigation of the human brain at cellular and microcircuit level remains challenging due to the fragile viability of neuronal tissue, inter- and intra-variability of the samples and limited availability of human brain material. Especially brain slices have proven to be an excellent source to investigate brain physiology and disease at cellular and small network level, overcoming the temporal limits of acute slices. Here we provide a revised, detailed protocol of the production and in-depth knowledge on long-term culturing of such human organotypic brain slice cultures for research purposes. We highlight the critical pitfalls of the culturing process of the human brain tissue and present exemplary results on viral expression, single-cell Patch-Clamp recordings, as well as multi-electrode array recordings as readouts for culture viability, enabling the use of organotypic brain slice cultures of these valuable tissue samples for basic neuroscience and disease modeling (Fig. 1).
Competing Interests: Declaration of Competing Interest All authors confirm that there are no relevant financial or non-financial competing interests to report.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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