SNAP-25, but not SNAP-23, is essential for photoreceptor development, survival, and function in mice.

Autor: Huang M; Division of Experimental & Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada., Chow CH; Division of Experimental & Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada., Gurdita A; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.; Donald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada., Harada H; Donald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada., Pham Truong VQB; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.; Donald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada., Eide S; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada., Sun HS; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.; Department of Anatomy, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada., Feng ZP; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada., Monnier PP; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada.; Donald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.; Department of Ophthalmology & Vision Sciences, University of Toronto, Toronto, ON, M5T 3A9, Canada., Wallace VA; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, M5S 1A8, Canada.; Donald K. Johnson Eye Institute, University Health Network, Toronto, ON, M5T 0S8, Canada.; Department of Ophthalmology & Vision Sciences, University of Toronto, Toronto, ON, M5T 3A9, Canada., Sugita S; Division of Experimental & Translational Neuroscience, Krembil Brain Institute, University Health Network, Toronto, ON, M5T 0S8, Canada. Shuzo.Sugita@uhnresearch.ca.; Department of Physiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada. Shuzo.Sugita@uhnresearch.ca.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Jan 05; Vol. 7 (1), pp. 34. Date of Electronic Publication: 2024 Jan 05.
DOI: 10.1038/s42003-023-05760-8
Abstrakt: SNARE-mediated vesicular transport is thought to play roles in photoreceptor glutamate exocytosis and photopigment delivery. However, the functions of Synaptosomal-associated protein (SNAP) isoforms in photoreceptors are unknown. Here, we revisit the expression of SNAP-23 and SNAP-25 and generate photoreceptor-specific knockout mice to investigate their roles. Although we find that SNAP-23 shows weak mRNA expression in photoreceptors, SNAP-23 removal does not affect retinal morphology or vision. SNAP-25 mRNA is developmentally regulated and undergoes mRNA trafficking to photoreceptor inner segments at postnatal day 9 (P9). SNAP-25 knockout photoreceptors develop normally until P9 but degenerate by P14 resulting in severe retinal thinning. Photoreceptor loss in SNAP-25 knockout mice is associated with abolished electroretinograms and vision loss. We find mistrafficked photopigments, enlarged synaptic vesicles, and abnormal synaptic ribbons which potentially underlie photoreceptor degeneration. Our results conclude that SNAP-25, but not SNAP-23, mediates photopigment delivery and synaptic functioning required for photoreceptor development, survival, and function.
(© 2024. The Author(s).)
Databáze: MEDLINE
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