| Autor: |
Avelino JA; Department of Comparative Medicine, Yale School of Medicine, New Haven, Connecticut., Walsh CA; Department of Comparative Medicine, Yale School of Medicine, New Haven, Connecticut., Wharton KN; Institute of Comparative Medicine, Columbia University, New York, New York., Ekanayake D; Department of Mathematics and Philosophy, Western Illinois University, Macomb, Illinois., Ekanayake-Alper D; Department of Comparative Medicine, Yale School of Medicine, New Haven, Connecticut;, Email: dil.ekanayake-alper@yale.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of the American Association for Laboratory Animal Science : JAALAS [J Am Assoc Lab Anim Sci] 2024 Mar 01; Vol. 63 (2), pp. 182-189. Date of Electronic Publication: 2024 Jan 05. |
| DOI: |
10.30802/AALAS-JAALAS-23-000064 |
| Abstrakt: |
Guinea pigs are often used in translational research, but providing them with safe and effective anesthesia is a challenge. Common methods like inhalant anesthesia and injectable ketamine/xylazine induce surgical anesthesia but can negatively affect cardiovascular, respiratory, and thermoregulatory systems and complicate the interpretation of research outcomes. Several alternative anesthetic regimens have been investigated, but none have consistently achieved a surgical plane of anesthesia. Therefore, identifying an anesthetic regimen that achieves a stable state of the surgical plane of anesthesia while preserving cardiorespiratory function would be a valuable contribution. To address this issue, we compared the efficacy of 3 anesthetic combinations in female Dunkin-Hartley guinea pigs: 1) alfaxalone, dexmedetomidine, and fentanyl (ADF); 2) alfaxalone, midazolam, and fentanyl (AMF); and 3) alfaxalone, midazolam, fentanyl, and isoflurane (AMFIso). We monitored anesthetic depth, heart rate, oxygenation, respiratory rate, respiratory effort, blood pressure, and body temperature every 15 min from injection to recovery. We also recorded the time to loss of righting reflex, duration of anesthesia, and time to achieve a surgical plane. The results showed no statistically significant differences in induction and recovery times among the groups. In the AMFIso group, 100% of the animals achieved a surgical plane of anesthesia, whereas only 10% of the animals in the AMF group reached that level. None of the animals in ADF group reached a surgical plane of anesthesia. Respiratory rate was significantly lower in the AMFIso as compared with the ADF group ( P < 0.001) but was not different between the AMF and ADF groups. Temperature was significantly lower in the AMFIso group as compared with both the ADF and AMF groups ( P < 0.001). In conclusion, both combinations of solely injectable anesthetics assessed in this study can be used for short, nonpainful procedures without significant cardiorespiratory depression. However, for mildly to moderately painful surgical procedures, the addition of an inhalant anesthetic like isoflurane is necessary for female guinea pigs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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