| Autor: |
Zhang X; Department of Microbiology, College of Life Sciences, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China., Wu N; Department of Microbiology, College of Life Sciences, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China., Ke Z; College of Rural Revitalization, Jiangsu Open University, Nanjing, Jiangsu 210036, PR China., Shi J; Department of Microbiology, College of Life Sciences, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China., Wang L; College of Rural Revitalization, Jiangsu Open University, Nanjing, Jiangsu 210036, PR China., Yuan C; College of Rural Revitalization, Jiangsu Open University, Nanjing, Jiangsu 210036, PR China., He J; Department of Microbiology, College of Life Sciences, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.; Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province, Nanjing, Jiangsu 210095, PR China. |
| Abstrakt: |
Dicamba is widely used in the paddy field to control broadleaf weeds. Dicamba easily migrates to deep soil, which is anoxic; however, the anaerobic catabolism of dicamba in paddy soil is still unknown. In this study, an anaerobic dicamba-degrading consortium was enriched from deep paddy soil. The consortium completely degraded 0.83 mM dicamba within 7 days. Five metabolites were identified, one of which is a new metabolite, 2,5-dichlorophenol, and a novel anaerobic dicamba degradation pathway was proposed. 2.5 mM dicamba, 1.5-2.0% NaCl, and 20 mM electron acceptors Na 2 SO 4 , NaNO 3 , and FeCl 3 , and 0.5 mM or more of metabolites 3-CP and 2,5-DCP strongly inhibited the degradation efficiency. During enrichment, the microbial community of the consortium was significantly changed with OTU numbers, and diversity decreased. The study is valuable to elucidate the catabolism and ecotoxicology studies of dicamba in paddy soil and to facilitate the engineering application of anaerobic technology to treat dicamba-manufacturing wastewater. |
