Development and characterization of an immortalized swine respiratory cell line for influenza A virus research.

Autor:	Neasham PJ; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., Pliasas VC; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., North JF; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States., Johnson C; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States., Tompkins SM; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States.; Center for Vaccines and Immunology, University of Georgia, Athens, GA, United States., Kyriakis CS; Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United States.; Emory-UGA Center of Excellence for Influenza Research and Surveillance (CEIRS), Atlanta, GA, United States.; Center for Vaccines and Immunology, University of Georgia, Athens, GA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Dec 18; Vol. 10, pp. 1258269. Date of Electronic Publication: 2023 Dec 18 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1258269
Abstrakt:	Introduction: Swine serve as an important intermediate host species for generating novel influenza A viruses (IAVs) with pandemic potential because of the host's susceptibility to IAVs of swine, human and avian origin. Primary respiratory cell lines are used in IAV research to model the host's upper respiratory tract in vitro . However, primary cell lines are limited by their passaging capacity and are time-consuming for use in industry and research pipelines. We were interested in developing and characterizing a biologically relevant immortalized swine respiratory cell line that could be used for efficient propagation and characterization of swine IAV isolates. Methods: Lung tissue for the generation of primary swine respiratory cells were isolated from the bronchi of an 8-week-old Yorkshire/Hampshire pig, which were immortalized by transduction of the SV40 T antigen using a lentivirus vector. The transduction of the SV40 T antigen was confirmed by Real Time RT-PCR in cells passaged greater than twenty times. Results: Immortalized swine respiratory cells expressed primarily α2,6 sialic acid receptors and were susceptible to both swine and human IAVs, with swine viruses exhibiting higher replication rates. Notably, infection with a swine H3N2 isolate prompted increased IL-6 and IL-1α protein secretion compared to a seasonal human H3N2 virus. Even after 20 passages, the immortalized cells maintained the primary respiratory cell phenotype and remained permissive to IAV infection without exogenous trypsin. Discussion: In summary, our developed immortalized swine respiratory cell line offers an alternative in vitro substrate for studying IAV replication and transmission dynamics in pigs, overcoming the limitations of primary respiratory cells in terms of low passage survivability and cost. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2023 Neasham, Pliasas, North, Johnson, Tompkins and Kyriakis.)
Databáze:	MEDLINE
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