Virulence profile of Candida spp. isolated from an anaerobic biodigester supplied with dairy cattle waste.

Autor: Branda Dos Reis C; Laboratory of Microorganism Cell Biology, Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: camilabrandareis@gmail.com., Otenio MH; Laboratory of Livestock Waste Management, Brazilian Agricultural Research Corporation - Dairy Cattle, Juiz de Fora, Minas Gerais, 36038-330, Brazil. Electronic address: marcelo.otenio@embrapa.br., Júnior AMM; Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, 36036-900, Brazil. Electronic address: andersonjrjf@gmail.com., Maia Dornelas JC; Laboratory of Mycology, Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: joaocmdo@yahoo.com.br., Fonseca do Carmo PH; Laboratory of Mycology, Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: paulofonsecca@gmail.com., Viana RO; Laboratory of Microorganism Cell Biology, Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: roberta.oliveiraviana@gmail.com., Santos Ricoy AC; Laboratory of Microorganism Cell Biology, Microbiology Department, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: carolricoy1993@hotmail.com., de Souza Alves V; Microbiology Department, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil. Electronic address: gouveiava@icb.ufmg.br.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Feb; Vol. 187, pp. 106516. Date of Electronic Publication: 2024 Jan 02.
DOI: 10.1016/j.micpath.2023.106516
Abstrakt: Anaerobic biodigesters play a crucial role in enhancing animal waste management. However, the presence of pathogens in the biodigestion process poses a significant concern. Candida spp., a widespread fungus known for its opportunistic nature and adaptability to diverse environmental conditions, including reciprocal transmission between humans and animals, is one such pathogen of concern. Therefore, it is imperative to assess the virulence profile of Candida spp. originating from anaerobic biodigestion processes. Here we demonstrate that strains isolated from the biodigestion process of dairy cattle waste exhibit noteworthy virulence mechanisms, surpassing the virulence of clinical control strains. After we identified strains from affluent, effluent, and biofertilizer, we observed that all analyzed isolates produced biofilm. Additionally, a substantial proportion of these isolates demonstrated phospholipase production, while only a few strains exhibited protease production. Furthermore, all strains exhibited resistance or dose-dependent responses to amphotericin B and itraconazole, with the majority displaying resistance to fluconazole. In the in vivo test, we observed a significant correlation (p < 0.05) between the LT50 and biofilm formation as well as hyphae/pseudohyphae production. Additionally, some isolates demonstrated a quicker nematode-killing capacity compared to clinical controls. Our findings underscore the considerable pathogenic potential of certain Candida species present in the dynamics of anaerobic biodigestion. Importantly, the anaerobic biodigester system did not eliminate Candida strains from dairy cattle waste, highlighting the need for caution in utilizing biodigester products. We advocate for further studies to explore the virulence of other microorganisms in various animal production contexts. Furthermore, our results emphasize the urgency of enhancing waste treatment methods to effectively eliminate pathogens and curb their potential dissemination.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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