Interaction of graphene and WS 2 with neutrophils and mesenchymal stem cells: implications for peripheral nerve regeneration.

Autor:	Convertino D; Center for Nanotechnology Innovation @ NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, Pisa, Italy. domenica.convertino@iit.it., Nencioni M; Department of Pharmacy, University of Pisa, Via Bonanno 6, Pisa, Italy. chiara.giacomelli@unipi.it., Russo L; Department of Pharmacy, University of Pisa, Via Bonanno 6, Pisa, Italy. chiara.giacomelli@unipi.it., Mishra N; Center for Nanotechnology Innovation @ NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, Pisa, Italy. domenica.convertino@iit.it.; Graphene Labs, Istituto Italiano di Tecnologia, Via Morego 30, Genova, Italy., Hiltunen VM; Center for Nanotechnology Innovation @ NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, Pisa, Italy. domenica.convertino@iit.it.; Graphene Labs, Istituto Italiano di Tecnologia, Via Morego 30, Genova, Italy., Bertilacchi MS; Department of Pharmacy, University of Pisa, Via Bonanno 6, Pisa, Italy. chiara.giacomelli@unipi.it., Marchetti L; Center for Nanotechnology Innovation @ NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, Pisa, Italy. domenica.convertino@iit.it.; Department of Pharmacy, University of Pisa, Via Bonanno 6, Pisa, Italy. chiara.giacomelli@unipi.it., Giacomelli C; Department of Pharmacy, University of Pisa, Via Bonanno 6, Pisa, Italy. chiara.giacomelli@unipi.it., Trincavelli ML; Department of Pharmacy, University of Pisa, Via Bonanno 6, Pisa, Italy. chiara.giacomelli@unipi.it., Coletti C; Center for Nanotechnology Innovation @ NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro 12, Pisa, Italy. domenica.convertino@iit.it.; Graphene Labs, Istituto Italiano di Tecnologia, Via Morego 30, Genova, Italy.
Jazyk:	angličtina
Zdroj:	Nanoscale [Nanoscale] 2024 Jan 25; Vol. 16 (4), pp. 1792-1806. Date of Electronic Publication: 2024 Jan 25.
DOI:	10.1039/d3nr04927b
Abstrakt:	Graphene and bidimensional (2D) materials have been widely used in nerve conduits to boost peripheral nerve regeneration. Nevertheless, the experimental and commercial variability in graphene-based materials generates graphene forms with different structures and properties that can trigger entirely diverse biological responses from all the players involved in nerve repair. Herein, we focus on the graphene and tungsten disulfide (WS 2 ) interaction with non-neuronal cell types involved in nerve tissue regeneration. We synthesize highly crystalline graphene and WS 2 with scalable techniques such as thermal decomposition and chemical vapor deposition. The materials were able to trigger the activation of a neutrophil human model promoting Neutrophil Extracellular Traps (NETs) production, particularly under basal conditions, although neutrophils were not able to degrade graphene. Of note is that pristine graphene acts as a repellent for the NET adhesion, a beneficial property for nerve conduit long-term applications. Mesenchymal stem cells (MSCs) have been proposed as a promising strategy for nerve regeneration in combination with a conduit. Thus, the interaction of graphene with MSCs was also investigated, and reduced viability was observed only on specific graphene substrates. Overall, the results confirm the possibility of regulating the cell response by varying graphene properties and selecting the most suitable graphene forms.
Databáze:	MEDLINE
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