Evaluation the cytotoxic effect of Fe 3 O 4 @Glu-Gingerol on lung adenocarcinoma cell line (A549) with biological mechanisms.

Autor: Alkinani TA; College of Pharmacy, Al-zahraa University for Women, Iraq., Bajgiran FA; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Rezaei M; Iranian Research Organization for Science and Technology, Tehran, Iran., Maivan AM; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Golrokh FJ; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Bejarbaneh M; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Mojdehi SR; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Gorji S; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Ghasemian R; Department of Medical Sciences, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran., Dashtban Jalil Pustin Sarai M; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Akbari F; Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Dehghan S; Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran., Mirzaee F; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran., Abdulrahman NH; College of Pharmacy, Al-zahraa University for Women, Iraq., Salehzadeh A; Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran.
Jazyk: angličtina
Zdroj: Heliyon [Heliyon] 2023 Dec 10; Vol. 10 (1), pp. e23419. Date of Electronic Publication: 2023 Dec 10 (Print Publication: 2024).
DOI: 10.1016/j.heliyon.2023.e23419
Abstrakt: The use of nanotechnology products with supermagnetic properties for targeted delivery of drugs has gained attention recently. Due to the anticancer features of Gingerol, the major phenolic compound from Ginger, this study aims to prepare Fe 3 O 4 @Glucose-Gingerol nanoparticles (NPs) and investigate their anticancer potential in a lung adenocarcinoma cell line. The physical and chemical features of the nanoparticles were investigated by FT-IR, XRD, zeta potential, DLS, EDS mapping, VSM, and electron microscope imaging. Cytotoxic effects of the nanoparticles for the A549 (lung adenocarcinoma) and MRC-5 (normal) cell lines was investigated by MTT assay. Furthermore, the effects of Fe 3 O 4 @Glucose-Gingerol nanoparticles on the expression of the CASP8 , BAX , and BCL2 genes and the activity of Caspase 3 were characterized. The flow cytometry assay (annexin V/PI) was employed to find out the percentage of apoptotic cells. The Fe 3 O 4 @Glu-Gingerol NPs were spherical (42-67 nm), without elemental impurity, and with surface charge, DLS size, and magnetic saturation of -47.7 mV, 154 nm, and 35 emu/g, respectively. Fe 3 O 4 @Glu-Gingerol NPs showed a remarkable greater toxicity in the A549 cells than normal cell line with the 50 % inhibition concentration (IC 50 ) of 190 and 554 μg/mL, respectively. Treatment of lung adenocarcinoma cells with the Fe 3 O 4 @Glu-Gingerol NPs led to an increase in cell apoptosis from 4.6 to 39.48 %. Also, the CASP8 and BAX genes were upregulated by 2.49 and 2.8 folds, respectively, while a downregulation by 0.75 folds was noticed for the BCL2 . Moreover, apoptotic features were observed in Fe 3 O 4 @Glu-Gingerol NPs treated cells by Hoechst staining, and activation of Caspase 3 by 2.8 folds. This study revealed that the Fe 3 O 4 @Glu-Gingerol NPs have antiproliferative effects on the lung adenocarcinoma cell line by activation of intrinsic and extrinsic apoptosis that is a promising feature in cancer treatment.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2023 The Authors. Published by Elsevier Ltd.)
Databáze: MEDLINE
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