Nanosensor detection of reactive oxygen and nitrogen species leakage in frustrated phagocytosis of nanofibres.

Autor: Qi YT; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Zhang FL; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Tian SY; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Wu HQ; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Zhao Y; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Zhang XW; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Liu YL; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China., Fu P; Institute of Surface-Earth System Science, School of Earth System Science, Tianjin University, Tianjin, People's Republic of China., Amatore C; State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, People's Republic of China. christian.amatore@ens.psl.eu.; PASTEUR, Département de Chimie, École Normale Supérieure, PSL Research University, Sorbonne University, Paris, France. christian.amatore@ens.psl.eu., Huang WH; College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China. whhuang@whu.edu.cn.; Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China. whhuang@whu.edu.cn.
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2024 Apr; Vol. 19 (4), pp. 524-533. Date of Electronic Publication: 2024 Jan 03.
DOI: 10.1038/s41565-023-01575-0
Abstrakt: Exposure to widely used inert fibrous nanomaterials (for example, glass fibres or carbon nanotubes) may result in asbestos-like lung pathologies, becoming an important environmental and health concern. However, the origin of the pathogenesis of such fibres has not yet been clearly established. Here we report an electrochemical nanosensor that is used to monitor and quantitatively characterize the flux and dynamics of reactive species release during the frustrated phagocytosis of glass nanofibres by single macrophages. We show the existence of an intense prolonged release of reactive oxygen and nitrogen species by single macrophages near their phagocytic cups. This continued massive leakage of reactive oxygen and nitrogen species damages peripheral cells and eventually translates into chronic inflammation and lung injury, as seen during in vitro co-culture and in vivo experiments.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
Externí odkaz: