Realistic microplastics harness bacterial presence and promote impairments in early zebrafish embryos: Behavioral, developmental, and transcriptomic approaches.

Autor: Missawi O; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium. Electronic address: omayma.missawi@unamur.be., Wouters C; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium., Lambert J; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium., Garigliany MM; University of Liege, Laboratory of Veterinary Pathology, Fundamental and Applied Research for Animals & Health (FARAH), Liege, Belgium., Kestemont P; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium., Cornet V; University of Namur, Research Unit in Environmental and Evolutionary Biology (URBE), Institute of Life, Earth & Environment, Namur, Belgium.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Feb; Vol. 350, pp. 141107. Date of Electronic Publication: 2024 Jan 01.
DOI: 10.1016/j.chemosphere.2023.141107
Abstrakt: The plastisphere is a newly recognized ecosystem. However, its interaction with early life stages of aquatic vertebrates is a multifaceted issue that requires further research. This study investigated the involvement of bacteria in shaping realistic microplastics hazards in zebrafish Danio rerio embryos. Fish were exposed to bottle micro-fragments (FR) and textile micro-fibers (FI) of polyethylene terephthalate (5-15 μm), concomitant with Aeromonas salmonicida achromogenes challenge from 2h post-fertilization for 3 days. Egg chorion showed affinity for FR and FI, inducing earlier embryo hatching. However, this effect was masked by biofilm invasion. Fragments were more detrimental than fibers on developmental parameters, while bacterial presence compromised body length, eye, and yolk sac surface area. In a further finding, MPs alone increased locomotor activity in zebrafish larvae, without synergistic effect when combined with bacteria. Data showed that realistic MPs had no significant effects except for downregulated sod and cyp1a gene expression, whereas bacterial challenge inhibited larval potency for most of the evaluated mRNA levels (mpx (immune system), apoeb (lipid metabolism), nfkb and tfa (inflammation), cyp and sod (oxidative stress)). This study provides new insights into realistic microplastic effects under relevant conditions when combined with environmental pathogen within the first life stages of aquatic vertebrates.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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