Characterization of genetic and molecular tools for studying the endogenous expression of Lactate dehydrogenase in Drosophila melanogaster.

Autor: Rai M; Department of Biology, Indiana University, Bloomington, IN, United States of America., Carter SM; Department of Biology, Indiana University, Bloomington, IN, United States of America., Shefali SA; Department of Biology, Indiana University, Bloomington, IN, United States of America., Chawla G; Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institute of Eminence (SNIoE), Dadri, Uttar Pradesh, India., Tennessen JM; Department of Biology, Indiana University, Bloomington, IN, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jan 03; Vol. 19 (1), pp. e0287865. Date of Electronic Publication: 2024 Jan 03 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0287865
Abstrakt: Drosophila melanogaster larval development relies on a specialized metabolic state that utilizes carbohydrates and other dietary nutrients to promote rapid growth. One unique feature of the larval metabolic program is that Lactate Dehydrogenase (Ldh) activity is highly elevated during this growth phase when compared to other stages of the fly life cycle, indicating that Ldh serves a key role in promoting juvenile development. Previous studies of larval Ldh activity have largely focused on the function of this enzyme at the whole animal level, however, Ldh expression varies significantly among larval tissues, raising the question of how this enzyme promotes tissue-specific growth programs. Here we characterize two transgene reporters and an antibody that can be used to study Ldh expression in vivo. We find that all three tools produce similar Ldh expression patterns. Moreover, these reagents demonstrate that the larval Ldh expression pattern is complex, suggesting the purpose of this enzyme varies across cell types. Overall, our studies validate a series of genetic and molecular reagents that can be used to study glycolytic metabolism in the fly.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Rai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje