Simplicispira sedimenti sp. nov., isolated from a sediment of drainage ditch in winery.

Autor: Chen X; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China., Li YD; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China., Liu XM; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China., Gao L; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China., Zhou XK; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China., Dong LM; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China., Du G; School of Life Sciences, Yunnan Minzu University, Kunming, 650031, People's Republic of China., Habib N; Department of Microbiology, Shaheed Benazir Bhutto Women University Peshawar, Peshawar, KPK, Pakistan., Li WJ; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China. liwenjun3@mail.sysu.edu.cn., Duan YQ; China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People's Republic of China. 88084159@qq.com.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2024 Jan 03; Vol. 117 (1), pp. 13. Date of Electronic Publication: 2024 Jan 03.
DOI: 10.1007/s10482-023-01899-w
Abstrakt: A Gram-stain-negative, motile (by single polar flagellum) and rod-shaped bacterium, designated W1-6 T , was isolated from a sediment of drainage ditch in winery in Guiyang, south-western China. Strain W1-6 T showed the highest 16S rRNA gene sequence similarities with the type strain of Acidovorax wautersii (98.1%) and Simplicispira lacusdiani (97.9%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W1-6 T was placed adjacent to the members of the genus Simplicispira and formed a separat subclade. Cells showed oxidase and catalase negative reactions. The only respiratory quinone detected was ubiquinone-8 (Q-8). Summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c), C 16:0 and summed feature 8 (C 18:1 ω7c and/or C 18:1 ω6c) were predominant cellular fatty acids (> 10%) of strain W1-6 T . Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and five unidentified phospholipids were found in the polar lipid extraction. The genomic DNA G + C content was 65.6%. Strain W1-6 T shared the highest digital DNA-DNA hybridization [dDDH, (27.6%)] and average nucleotide identity [ANI (84.3%)] values with the type strain of S. lacusdiani. The dDDH and ANI values were below the cutoff level (dDDH 70%; ANI 95-96%) for species delineation. The polyphasic characteristics indicated that the strain W1-6 T represents a novel species of the genus Simplicispira, for which the name Simplicispira sedimenti sp. nov. is proposed. The type strain is W1-6 T (= CGMCC 1.16274 T  = NBRC 115624 T ).
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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