| Autor: |
Gupta A; Plant Biotechnology, Department of Genetics and Plant Breeding, Banaras Hindu University, Mirzapur, India., Mardi P; Plant Biotechnology, Department of Genetics and Plant Breeding, Banaras Hindu University, Mirzapur, India., Mishra PKK; Unit of Teaching Veterinary Clinical Complex, Faculty of Veterinary and Animal Sciences, Banaras Hindu University, Mirzapur, India., Kumar A; Department of Animal Genetics and Breeding, Faculty of Veterinary and Animal Sciences, Banaras Hindu University, Mirzapur, India., Kumar R; Plant Biotechnology, Department of Genetics and Plant Breeding, Banaras Hindu University, Mirzapur, India., Mahapatra A; Department of Veterinary Anatomy, Faculty of Veterinary and Animal Sciences, Banaras Hindu University, Mirzapur, India., Jena A; Fisheries and Animal Resource Development Department, Bhubaneswar, India., Behera PC; Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, OUAT, Bhubaneshwar, India. |
| Abstrakt: |
In growing plant population, effect of stress is a perturb issue affecting its physiological, biochemical, yield loss and developmental growth. Protein-L-isoaspartate-O-methyltransferase (PIMT) is a broadly distributed protein repair enzyme which actuate under stressful environment or aging. Stress can mediate damage converting protein bound aspartate (Asp) residues to isoaspartate (iso-Asp). This spontaneous and deleterious conversion occurs at an elevated state of stress and aging. Iso-Asp formation is associated with protein inactivation and compromised cellular survival. PIMT can convert iso-Asp back to Asp, thus repairing and contributing to cellular survival. The present work describes the isolation, cloning, sequencing and expression of PIMT genes of Carica papaya ( Cp pimt ) and Ricinus communis ( Rc pimt ) Using gene specific primers, both the pimts were amplified from their respective cDNAs and subsequently cloned in prokaryotic expression vector pProEXHTa. BL21(DE3) strain of E. coli cells were used as expression host. The expression kinetics of both the PIMTs were studied with various concentrations of IPTG and at different time points. Finally, the PIMT supplemented BL21(DE3) cells were evaluated against different stresses in comparison to their counterparts with the empty vector control. |
