Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant ( pind ) in eggplant ( Solanum melongena L.).

Autor:	Luo L; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Molthoff J; Plant Breeding, Wageningen University and Research, Wageningen, Netherlands., Li Q; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Liu Y; Horticulture and Product Physiology, Wageningen University and Research, Wageningen, Netherlands., Luo S; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Li N; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Xuan S; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Wang Y; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Shen S; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Bovy AG; Plant Breeding, Wageningen University and Research, Wageningen, Netherlands., Zhao J; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China., Chen X; State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, International Joint R & D Center of Hebei Province in Modern Agricultural Biotechnology, College of Horticulture, Hebei Agricultural University, Baoding, China.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Dec 11; Vol. 14, pp. 1282661. Date of Electronic Publication: 2023 Dec 11 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1282661
Abstrakt:	Eggplant ( Solanum melongena L.) is a highly nutritious and economically important vegetable crop. However, the fruit peel of eggplant often shows poor coloration owing to low-light intensity during cultivation, especially in the winter. The less-photosensitive varieties produce anthocyanin in low light or even dark conditions, making them valuable breeding materials. Nevertheless, genes responsible for anthocyanin biosynthesis in less-photosensitive eggplant varieties are not characterized. In this study, an EMS mutant, named purple in the dark ( pind ), was used to identify the key genes responsible for less-photosensitive coloration. Under natural conditions, the peel color and anthocyanin content in pind fruits were similar to that of wildtype '14-345'. The bagged pind fruits were light purple, whereas those of '14-345' were white; and the anthocyanin content in the pind fruit peel was significantly higher than that in '14-345'. Genetic analysis revealed that the less-photosensitive trait was controlled by a single dominant gene. The candidate gene was mapped on chromosome 10 in the region 7.72 Mb to 11.71 Mb. Thirty-five differentially expressed genes, including 12 structural genes, such as CHS , CHI , F3H , DFR , ANS , and UFGT , and three transcription factors MYB113 , GL3 , and TTG2 , were identified in pind using RNA-seq. Four candidate genes EGP21875 (myb domain protein 113), EGP21950 (unknown protein), EGP21953 (CAAX amino-terminal protease family protein), and EGP21961 (CAAX amino-terminal protease family protein) were identified as putative genes associated with less-photosensitive anthocyanin biosynthesis in pind . These findings may clarify the molecular mechanisms underlying less-photosensitive anthocyanin biosynthesis in eggplant. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Luo, Molthoff, Li, Liu, Luo, Li, Xuan, Wang, Shen, Bovy, Zhao and Chen.)
Databáze:	MEDLINE
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