Synthesis and analysis of silica nanocarriers for pectinase immobilization: Enhancing enzymatic stability for continuous industrial applications.

Autor:	Behram T; Department of Biotechnology, Shaheed Benazir Bhutto University Sheringal Dir (Upper), KPK, Pakistan., Pervez S; Department of Biochemistry, Shaheed Benazir Bhutto Women University Peshawar, KPK, Pakistan., Nawaz MA; Department of Biotechnology, Shaheed Benazir Bhutto University Sheringal Dir (Upper), KPK, Pakistan., Ullah R; Department of Biotechnology, Shaheed Benazir Bhutto University Sheringal Dir (Upper), KPK, Pakistan., Khan AA; Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia., Ahmad B; Department of Biochemistry, Shaheed Benazir Bhutto Women University Peshawar, KPK, Pakistan., Alanzai AM; Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia., Ahmad A; Center for Sickle Cell Disease, College of Medicine, Howard University Washington DC, USA., Jan AK; Department of Chemistry, Shaheed Benzir Bhutto University Sheringal Dir (Upper), Pakistan., Rahman HU; Department of Chemistry, University of Turbat, Kech Baluchistan, Pakistan., Jamal M; Department of Microbiology, Abdul Wali Khan University Mardan, Pakistan., Tour Jan; Department of Botany, University of Malakand, Chakdara, Pakistan., Mohyuddin A; Department of Chemistry, The Emerson University Multan, Pakistan., Khan NM; Department of Agriculture, Shaheed Benazir Bhutto University Sheringal Dir (Upper), KPK, Pakistan., Ahmad S; Department of Pharmacy, Shaheed Benazir Bhutto University Sheringal Dir (Upper), KPK, Pakistan.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Dec 09; Vol. 10 (1), pp. e23549. Date of Electronic Publication: 2023 Dec 09 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2023.e23549
Abstrakt:	Pectinolytic enzymes are among the important group of industrial enzymes that have wide applications in different food industries. In this study, pectinase-based silica nanocarriers were synthesized using co-precipitation and cross-linking techniques. The resulting silica nanoparticles were investigated using scanning electron microscopy (SEM), energy-dispersive electron microscopy (EDEX), and X-ray diffraction (XRD) for determination of its morphology, elemental composition, and crystalline pattern. Under the optimal immobilization conditions like 1.5 % glutaraldehyde, 3000 IU/mg pectinase concentration, 90 min immobilization time and 40 °C immobilization temperature, pectinase showed maximum immobilization yield. The immobilization of pectinase onto the silica nanocarriers led to enhanced catalytic characteristics, displaying higher enzymatic activity across various temperature and pH levels compared to soluble pectinase. Moreover, the immobilization substantially improved the temperature stability of pectinase, exhibiting 100 % of its initial activity even after 120 h of pre-incubation at 50 °C. Additionally, the silica nanocarrier pectinase retained 100 % of its original activity even after being reused 10 times in a single batch of reactions. These findings indicate that the immobilization of silica nanocarriers effectively enhances pectinase's industrial capabilities, making it economically feasible for industrial use and an efficient system for various biotechnological applications. Competing Interests: The authors declare no conflict of interest. (© 2023 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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