Extracellular vesicles from obese and diabetic mouse plasma alter C2C12 myotube glucose uptake and gene expression.

Autor: Pitzer CR; Center for Muscle, Metabolism and Neuropathology, Division of Regenerative and Rehabilitation Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Department of Physiology, College of Medicine, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Integrated Biomedical Sciences Graduate Program, College of Graduate Health Sciences, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Laboratory of Muscle Biology and Sarcopenia, Department of Physical Therapy, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA., Paez HG; Center for Muscle, Metabolism and Neuropathology, Division of Regenerative and Rehabilitation Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Department of Physiology, College of Medicine, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Integrated Biomedical Sciences Graduate Program, College of Graduate Health Sciences, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Laboratory of Muscle Biology and Sarcopenia, Department of Physical Therapy, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA., Ferrandi PJ; Center for Muscle, Metabolism and Neuropathology, Division of Regenerative and Rehabilitation Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Integrated Biomedical Sciences Graduate Program, College of Graduate Health Sciences, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Laboratory of Muscle and Nerve, Department of Diagnostic and Health Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA., Mohamed JS; Center for Muscle, Metabolism and Neuropathology, Division of Regenerative and Rehabilitation Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Laboratory of Muscle and Nerve, Department of Diagnostic and Health Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Tennessee Institute of Regenerative Medicine, The University of Tennessee Health Science Center, Memphis, Tennessee, USA., Alway SE; Center for Muscle, Metabolism and Neuropathology, Division of Regenerative and Rehabilitation Sciences, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Department of Physiology, College of Medicine, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Laboratory of Muscle Biology and Sarcopenia, Department of Physical Therapy, College of Health Professions, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.; Tennessee Institute of Regenerative Medicine, The University of Tennessee Health Science Center, Memphis, Tennessee, USA.
Jazyk: angličtina
Zdroj: Physiological reports [Physiol Rep] 2024 Jan; Vol. 12 (1), pp. e15898.
DOI: 10.14814/phy2.15898
Abstrakt: Recent studies have indicated a role for circulating extracellular vesicles (EVs) in the pathogenesis of multiple diseases. However, most in vitro studies have used variable and arbitrary doses of EVs rather than interpreting EVs as an existing component of standard skeletal muscle cell culture media. The current study provides an initial investigation into the effects of circulating EVs on the metabolic phenotype of C2C12 myotubes by replacing EVs from fetal bovine serum with circulating EVs from control mice or mice with obesity and type 2 diabetes (OT2D). We report that EVs associated with OT2D decrease 2-NBDG uptake (a proxy measure of glucose uptake) in the insulin-stimulated state compared to controls. OT2D associated EV treatment also significantly decreased myosin heavy chain type 1 (MHCI) mRNA abundance in myotubes but had no effect on mRNA expression of any other myosin heavy chain isoforms. OT2D-associated circulating EVs also significantly increased lipid accumulation within myotubes without altering the expression of a selection of genes important for lipid entry, synthesis, or catabolism. The data indicate that, in a severely diabetic state, circulating EVs may contribute to insulin resistance and alter gene expression in myotubes in a manner consistent with the skeletal muscle phenotype observed in OT2D.
(© 2024 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)
Databáze: MEDLINE
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