Alveolar macrophage-expressed Plet1 is a driver of lung epithelial repair after viral pneumonia.

Autor: Pervizaj-Oruqaj L; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany., Selvakumar B; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), Buenos Aires, Argentina., Ferrero MR; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), Buenos Aires, Argentina., Heiner M; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany., Malainou C; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany., Glaser RD; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Biomedical Informatics and Systems Medicine, Justus Liebig University, Giessen, Germany., Wilhelm J; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; Department of Internal Medicine II, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany., Bartkuhn M; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Biomedical Informatics and Systems Medicine, Justus Liebig University, Giessen, Germany., Weiss A; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; Department of Internal Medicine II, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany., Alexopoulos I; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany., Witte B; Department of General and Thoracic Surgery, University Hospital of Giessen, Giessen, Germany., Gattenlöhner S; Department of Pathology, University Hospital of Giessen, Giessen, Germany., Vadász I; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; Department of Internal Medicine II, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany., Morty RE; Department of Translational Pulmonology and the Translational Lung Research Center, University Hospital Heidelberg, Member of the German Center for Lung Research (DZL), Heidelberg, Germany., Seeger W; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.; Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), Buenos Aires, Argentina.; Department of Internal Medicine II, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany., Schermuly RT; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; Department of Internal Medicine II, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany., Vazquez-Armendariz AI; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany.; University of Bonn, Transdisciplinary Research Area Life and Health, Organoid Biology, Life & Medical Sciences Institute, Bonn, Germany., Herold S; Department of Internal Medicine V, Universities of Giessen and Marburg Lung Center, University Hospital Giessen, Justus Liebig University, Member of the German Center for Lung Research (DZL), Giessen, Germany. Susanne.herold@innere.med.uni-giessen.de.; Institute for Lung Health (ILH), Justus Liebig University, Giessen, Germany. Susanne.herold@innere.med.uni-giessen.de.; Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany. Susanne.herold@innere.med.uni-giessen.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Jan 02; Vol. 15 (1), pp. 87. Date of Electronic Publication: 2024 Jan 02.
DOI: 10.1038/s41467-023-44421-6
Abstrakt: Influenza A virus (IAV) infection mobilizes bone marrow-derived macrophages (BMDM) that gradually undergo transition to tissue-resident alveolar macrophages (TR-AM) in the inflamed lung. Combining high-dimensional single-cell transcriptomics with complex lung organoid modeling, in vivo adoptive cell transfer, and BMDM-specific gene targeting, we found that transitioning ("regenerative") BMDM and TR-AM highly express Placenta-expressed transcript 1 (Plet1). We reveal that Plet1 is released from alveolar macrophages, and acts as important mediator of macrophage-epithelial cross-talk during lung repair by inducing proliferation of alveolar epithelial cells and re-sealing of the epithelial barrier. Intratracheal administration of recombinant Plet1 early in the disease course attenuated viral lung injury and rescued mice from otherwise fatal disease, highlighting its therapeutic potential.
(© 2024. The Author(s).)
Databáze: MEDLINE
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