Characterization and biological applications of gonadal extract of Paracentrotus lividus collected along the Mediterranean coast of Alexandria, Egypt.

Autor: Nagy NS; Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt., Essawy AE; Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt., Al-Sherif SS; Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt., Ali MM; Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden., Alsawy ES; Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt., Helal M; National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jan 02; Vol. 19 (1), pp. e0296312. Date of Electronic Publication: 2024 Jan 02 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0296312
Abstrakt: Marine invertebrates represent a valuable reservoir of pharmaceutical bioactive compounds with potential relevance to various medical applications. These compounds exhibit notable advantages when compared to their terrestrial counterparts, in terms of their potency, activity, and mechanism of action. Within this context, the present work aimed to extract, chemically characterize, and investigate the bioactivity of the gonadal extract of the sea urchin Paracentrotus lividus (P. lividus) collected along the Mediterranean coast of Alexandria, Egypt. Fractions of the gonadal extract were characterized by Spectrophotometry and gas chromatography-mass spectrometry (GC-MS), and their bioactivities were investigated in vitro. The analysis supported the extract richness of carotenoids and bioactive compounds. The extract showed promising anticancer activity against three different breast cancer cell lines with different levels of aggressiveness and causative factors, namely MDA-MB-231, MDA-MB-453, and HCC-1954. Gene expression analysis using RT-qPCR showed that P. lividus extract inhibited the expression of crucial factors involved in cell cycle regulation and apoptosis. In addition, the extract significantly inhibited the lipo-polysaccharides (LPS) induced inflammation in the RAW264.7 macrophage cell line and exerted anti-bacterial activity against the Gram-negative bacteria Klebsiella pneumoniae and Pseudomonas aeruginosa. Collectively, these results demonstrated the chemical richness and the wide-scale applicability of P. lividus gonadal extract as an anti-cancer, anti-bacterial, and anti-inflammatory natural extract.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Nagy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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