Prevalence and circulant genotypes of Chlamydia trachomatis in university women from cities in the Brazilian Amazon.

Autor: Dos Santos LM; Tropical Medicine Center, Federal University of Pará, Belém, Pará, Brazil., Vieira MRMDS; Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil., Vieira RC; Tropical Medicine Center, Federal University of Pará, Belém, Pará, Brazil., Silva LBDL; Tropical Medicine Center, Federal University of Pará, Belém, Pará, Brazil., de Macêdo GMM; Tropical Medicine Center, Federal University of Pará, Belém, Pará, Brazil., Miranda AE; Department of Social Medicine, Health Sciences Center, Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil., Brasiliense DM; Section of Bacteriology and Mycology, Evandro Chagas Institute, Ananindeua, Pará, Brazil., E Guimarães RJPS; Geoprocessing Laboratory, Instituto Evandro Chagas, Ananindeua, Pará, Brazil., Sousa EC Junior; Parasitology Section, Instituto Evandro Chagas, Ananindeua, Pará, Brazil., Ferrari SF; Department of Ecology, Federal University of Sergipe, São Cristóvão, Brazil., Pinheiro HHC; Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil., Ishikawa EAY; Tropical Medicine Center, Federal University of Pará, Belém, Pará, Brazil., de Sousa MS; Tropical Medicine Center, Federal University of Pará, Belém, Pará, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jan 02; Vol. 19 (1), pp. e0287119. Date of Electronic Publication: 2024 Jan 02 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0287119
Abstrakt: Background: Approximately 80% of infected women infected by Chlamydia trachomatis are asymptomatic, although this infection can lead to serious complications in the female reproductive tract. Few data on Chlamydia infection and genotypes are available in Amazonian communities.
Objectives: To describe the prevalence of and associated factors and to identify the genotypes of sexual C. trachomatis infection in female university students in different urban centers (capital and interiors) in the Brazilian state of Pará, in the eastern Amazon region.
Methods: A cross-sectional study was performed among young women attending public universities in four different urban centers in the eastern Amazon region. They were invited to participate in the studt and cervical secretions were collected for molecular diagnosis of C. trachomatis. We utilized amplification of the ompA gene by nested PCR. Positive samples were genotyped by nucleotide sequencing. Study participants completed a questionnaire on social, epidemiological, and reproductive health variables. A Qui-square and Binominal regression test were used to evaluate the degree of association of these variables with the infection.
Results: A total of 686 female students was included in the study. The overall prevalence of C. trachomatis was 11.2% (77/686). The prevalence of this infection was higher in interiors (15.2% vs 9.5%/ p: 0.0443). Female university students who do not have a sexual partner (11.8%/p <0.008), who do not use a condom in their sexual relations (17.8%/p <0.0001) and who reported having suffered a miscarriage (32%/p <0.0001) have high chances of acquiring this sexual infection. The ompA gene was sequenced in only 33 (42.8%) samples, revealing the genotype J was the most frequent (27.2% [9/33]), followed by genotypes D (24.2% [8/33]), and then genotypes F (18.2% [6/33]), E (15.1% [5/33]) K (6.1% [2/33]), Ia (6.1% [2/33]), and G (3.1% [1/33]).
Conclusions: The high prevalence of sexual infection by C. trachomatis in the female university students from the interior of the state of Pará, individuals with no fixed sexual partner, those that had had a miscarriage, the students that do not use condoms in their sexual relations. The genotype J of C. trachomatis genotypes was the most frequent. These data are important to help defining the epidemiological effects of chlamydial infections in Amazonian populations.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 dos Santos et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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