Acute and persistent effects of oral glutamine supplementation on growth, cellular proliferation, and tight junction protein transcript abundance in jejunal tissue of low and normal birthweight pre-weaning piglets.

Autor: Schregel J; Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology, Dummerstorf, Germany., Schulze Holthausen J; Department of Veterinary Medicine, Institute of Animal Nutrition, Freie Universität Berlin, Berlin, Germany., Sciascia QL; Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology, Dummerstorf, Germany., Görs S; Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology, Dummerstorf, Germany., Li Z; Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology, Dummerstorf, Germany., Tuchscherer A; Research Institute for Farm Animal Biology (FBN), Institute of Genetics and Biometry, Dummerstorf, Germany., Albrecht E; Institute of Muscle Biology and Growth, Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany., Zentek J; Department of Veterinary Medicine, Institute of Animal Nutrition, Freie Universität Berlin, Berlin, Germany., Metges CC; Research Institute for Farm Animal Biology (FBN), Institute of Nutritional Physiology, Dummerstorf, Germany.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jan 02; Vol. 19 (1), pp. e0296427. Date of Electronic Publication: 2024 Jan 02 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0296427
Abstrakt: Breeding for higher fertility has resulted in a higher number of low birthweight (LBW) piglets. It has been shown that LBW piglets grow slower than normal birthweight (NBW) littermates. Differences in growth performance have been associated with impaired small intestinal development. In suckling and weaning piglets, glutamine (Gln) supplementation has been associated with improved growth and intestinal development. This study was designed to examine the effects of oral Gln supplementation on growth and small intestinal parameters in LBW and NBW suckling piglets. At birth (day 0), a total of 72 LBW (1.10 ± 0.06 kg) and 72 NBW (1.51 ± 0.06) male piglets were selected. At day 1, litters were standardized to 12 piglets, and experimental piglets supplemented daily with either Gln (1 g/kg BW) or isonitrogenous amounts of Alanine (Ala) as control (1.22 g/kg BW) until day 12. Creep feed was offered from day 14 onward. Subgroups of piglets were euthanized at days 5, 12, and 26 for the analyses of jejunal morphometry, cellular proliferation, glutathione concentration and transcript abundance of tight junction proteins. From age day 11 to 21, Gln supplemented LBW (LBW-Gln) piglets were heavier than Ala supplemented LBW (LBW-Ala) littermates (P = 0.034), while NBW piglets were heavier until age day 26 compared to LBW littermates. Villus height was higher in LBW-Gln compared to LBW-Ala on age day 12 (P = 0.031). Sporadic differences among supplementation and birthweight groups were detected for jejunal cellular proliferation, cellular population and glutathione concentration, whereas age was the most dominant factor. These results show that Gln supplementation improved the growth of LBW piglets compared to LBW-Ala beyond the termination of Gln supplementation, but this was not associated with consistent effects on selected parameters of jejunal development.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Schregel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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