Nanocellulose/Fe 3 O 4 /Ag Nanozyme with Robust Peroxidase Activity for Enhanced Antibacterial and Wound Healing Applications.

Autor: Geleto SA; Industrial Chemistry Department, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia., Ariti AM; Industrial Chemistry Department, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia., Gutema BT; Biotechnology Department, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia., Abda EM; Biotechnology Department, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia.; Bioprocess and Biotechnology Center of Excellence, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia., Abiye AA; Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa 1647, Ethiopia., Abay SM; Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa 1647, Ethiopia., Mekonnen ML; Industrial Chemistry Department, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia.; Nanotechnology Center of Excellence, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia., Workie YA; Industrial Chemistry Department, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia.; Nanotechnology Center of Excellence, Addis Ababa Science and Technology University, Addis Ababa 1647, Ethiopia.
Jazyk: angličtina
Zdroj: ACS omega [ACS Omega] 2023 Dec 14; Vol. 8 (51), pp. 48764-48774. Date of Electronic Publication: 2023 Dec 14 (Print Publication: 2023).
DOI: 10.1021/acsomega.3c05748
Abstrakt: Peroxidase memetic nanozymes with their free radical-mediated catalytic actions proved as efficacious antibacterial agents for combating bacterial resistance. Herein, nanocellulose (NC) extracted from Eragrostis teff straw was used to prepare NC/Fe 3 O 4 /Ag peroxidase nanozyme as an antibacterial and wound healing agent. Characterization of the nanozyme with XRD, FTIR, SEM-EDX, and XPS confirmed the presence of silver NPs and the magnetite phase of iron oxide dispersed on nanocellulose. The peroxidase activity of the prepared nanozyme was examined using TMB and H 2 O 2 as substrates which turned blue in acidic pH (λ max = 652 nm). With a lower K m (0.387 mM), the nanozyme showed a comparable affinity for TMB with that reported for the HRP enzyme. Furthermore, the nanozyme remained efficient over a broader temperature range while maintaining 61.53% of its activity after the fourth cycle. In vitro, antibacterial tests against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacterial strains showed that NC/Fe 3 O 4 /Ag exhibits concentration-dependent and enhanced antibacterial effect for Escherichia coli compared to NC and NC-Fe 3 O 4 and negative control. Furthermore, the wound-healing performance of the NC-Fe 3 O 4 -Ag nanozyme was investigated in vivo using an animal model (mice). The nanozyme showed 30% higher wound healing performance compared to the control base ointment and is comparable with the commercial nitrofurazone ointment. The results show the potential of the prepared nanozyme for wound-healing purposes.
Competing Interests: The authors declare no competing financial interest.
(© 2023 The Authors. Published by American Chemical Society.)
Databáze: MEDLINE
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