Protective role of Cecropin AD against LPS-induced intestinal mucosal injury in chickens.

Autor:	Zhi Y; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China., Li T; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China., Li Y; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China., Zhang T; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China., Du M; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China., Zhang Q; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China., Wang X; Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, the Key Laboratory of Otolaryngology-Head and Neck Surgery (Ministry of Education of China), Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China., Hu G; College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2023 Dec 14; Vol. 14, pp. 1290182. Date of Electronic Publication: 2023 Dec 14 (Print Publication: 2023).
DOI:	10.3389/fimmu.2023.1290182
Abstrakt:	Introduction: Cecropin AD (CAD), a renowned antimicrobial peptide, has shown promising potential in treating various bacterial infections. This study investigates the protective effects of CAD against lipopolysaccharide (LPS)-induced intestinal adversities in chickens. Methods: Sixty SPF-grade chicks were divided into groups and exposed to different dosages of CAD, followed by LPS administration. The study assessed the impact of CAD on intestinal mucosal injury markers, oxidative stress, and inflammation. Results: LPS significantly increased Diamine oxidase (DAO) and D-lactate (D-LA) levels, both indicators of intestinal mucosal injury. CAD treatment substantially attenuated these elevations, particularly at higher dosages. Additionally, CAD markedly reduced oxidative stress in intestinal tissues, as shown by normalized antioxidant levels and decreased reactive oxygen species. Histological analysis supported these findings, showing better-preserved villi structures in CAD-treated groups. Furthermore, CAD significantly reduced IL-6 and IL-8 expression post-LPS stimulation and effectively regulated the NLRP3 inflammasome pathway, decreasing associated factors like NLRP3, Caspase-1, IL-1b, and IL-18. Discussion: The study demonstrates CAD's therapeutic potential in alleviating LPS-induced intestinal injuries. The protective effects are primarily attributed to its anti-inflammatory and antioxidative actions and modulation of the NLRP3 inflammasome pathway. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Zhi, Li, Li, Zhang, Du, Zhang, Wang and Hu.)
Databáze:	MEDLINE
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