Electrophoresis, a transport technology that transitioned from moving boundary method to zone method.

Autor: Arakawa T; Alliance Protein Laboratories, 13380 Pantera Rd., San Diego, CA, 92130, USA. tarakawa2@aol.com., Nakagawa M; Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-Shi, Ibaraki, 318-0004, Japan., Sakuma C; Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-Shi, Ibaraki, 318-0004, Japan., Tomioka Y; Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-Shi, Ibaraki, 318-0004, Japan., Kurosawa Y; Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-Shi, Ibaraki, 318-0004, Japan., Ejima D; Sysmex Corporation, Technology Innovation, 1548 Shimo-Okutomi, Sayama, Saitama, 350-1332, Japan., Akuta T; Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-Shi, Ibaraki, 318-0004, Japan.
Jazyk: angličtina
Zdroj: European biophysics journal : EBJ [Eur Biophys J] 2024 Feb; Vol. 53 (1-2), pp. 1-13. Date of Electronic Publication: 2023 Dec 30.
DOI: 10.1007/s00249-023-01694-5
Abstrakt: Gel electrophoresis, a transport technology, is one of the most widely used experimental methods in biochemical and pharmaceutical research and development. Transport technologies are used to determine hydrodynamic or electrophoretic properties of macromolecules. Gel electrophoresis is a zone technology, where a small volume of sample is applied to a large separation gel matrix. In contrast, a seldom-used electrophoresis technology is moving boundary electrophoresis, where the sample is present throughout the separation phase or gel matrix. While the zone method gives peaks of separating macromolecular solutes, the moving boundary method gives a boundary between solute-free and solute-containing phases. We will review electrophoresis as a transport technology of zone and moving boundary methods and describe its principles and applications.
(© 2023. European Biophysical Societies' Association.)
Databáze: MEDLINE
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