Growth and productivity of Haematococcus pluvialis and Coelastrella saipanensis by photosystem modulation for understanding the heterotrophic nutritional strategy for bioremediation application.

Autor: Nayana K; Microalgae Group, Phycoscience Lab, Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, 671 320, Kasaragod, Kerala, India. Electronic address: nayanathanal@gmail.com., Babu VS; Microalgae Group, Phycoscience Lab, Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, 671 320, Kasaragod, Kerala, India. Electronic address: vaishnavsbabu963@gmail.com., Vidya D; Microalgae Group, Phycoscience Lab, Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, 671 320, Kasaragod, Kerala, India. Electronic address: vidyadvijaya@gmail.com., Sudhakar MP; Marine Biotechnology Division, National Institute of Ocean Technology, Ministry of Earth Sciences, Govt. of India, Pallikaranai, Chennai, 600100, Tamil Nadu, India; Marine Biopolymers & Advanced Bioactive Materials Research Lab, Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (Saveetha University), Chennai, 600 077, Tamil Nadu, India. Electronic address: mpsdhkr@gmail.com., Arunkumar K; Microalgae Group, Phycoscience Lab, Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, 671 320, Kasaragod, Kerala, India. Electronic address: arunnir@yahoo.co.in.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2024 Mar 15; Vol. 245, pp. 118077. Date of Electronic Publication: 2023 Dec 29.
DOI: 10.1016/j.envres.2023.118077
Abstrakt: In this study, Haematococcus pluvialis and Coelastrella saipanensis were evaluated for heterotrophic nutrition potential in dairy waste medium by blocking the PSII using DCMU. The study was done by four sets of experiments. In the first set, in the different concentrations DCMU-treatments, 20μL showed pronounced effect in H. pluvialis and C. saipanensis as 89 % and 83% decrease in cells (>30 and > 250 cells/mL) compared to control (536 ± 12.35 × 10 4 and 1167 ± 15.35 × 10 4  cells/mL, respectively). Damage to the PS II by DCMU interrupted the growth, which in turn produced a significant drop in the number of cells. In the second round of experiment, growth of algae in various dairy waste concentrations suggest that dairy wastewater (DWW) provides enough nutrients to produce 35.71 % and 64.74 % more cells in H. pluvialis and C. saipanensis, respectively compared to the control. In the third set, high DCMU concentration was added to microalgae cultures in DWW to assess the heterotrophic nutrition potential. Growth in cell number 34.4 ± 19 and 617.46 ± 60.44 cells/mL was recorded in H. pluvialis and C. saipanensis when grown control medium whereas addition of DCMU reduced the cell number to 1.53 ± 0.75 and 55.13 ± 0.75 cells/mL on 15 th day, respectively. This shows cells in cultures treated with DCMU reveal that algae can sustain their metabolic activity by utilizing the nutrients of dairy waste inhibiting photosystem. Fourth round of experiments found that microalgae could resume their growth and productivity by adapting to heterotrophic nutritional behaviour when DCMU given in mild dose at different time interval. This study conclude as C. saipanensis grows more readily by absorbing dairy waste nutrients than H. pluvialis. Therefore, C. saipanensis is an excellent choice for wastewater treatment through sustainable environmentally benign process after scale-up investigation. These results provide useful information to advance to molecular study for measuring microalgae's capability for bioremediation application.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kulanthaiyesu Arunkumar reports financial support was provided by Central University of Kerala. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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