Determination of the minimum infective dose of viral hemorrhagic septicemia virus (VHSV) in juvenile olive flounder, Paralichthys olivaceus using an immersion challenge model.

Autor: Yoon SY; Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea., Jang YS; Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea., Kim SJ; Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea; Pathology Division, National Institute of Fisheries Science (NIFS), Busan, Republic of Korea., Krishnan R; Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea; Department of Aquatic Animal Health Management, Faculty of Fisheries, Kerala University of Fisheries and Ocean Studies, Kerala, India., Oh MJ; Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea. Electronic address: ohmj@jnu.ac.kr.
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 2024 Feb; Vol. 340, pp. 199305. Date of Electronic Publication: 2023 Dec 29.
DOI: 10.1016/j.virusres.2023.199305
Abstrakt: Viral hemorrhagic septicemia virus (VHSV) affects over 80 fish species, leading to viral hemorrhagic septicemia (VHS). Horizontal VHSV transmission is widely studied, with researchers utilizing various doses to establish infection models. Infected hosts shed the virus into the environment, elevating the risk of transmission to naïve fish within the same system. This study aimed to ascertain the minimum infective dose of VHSV in olive flounder (Paralichthys olivaceus). In olive flounder, the detection of VHSV within the kidney exhibited the highest infection rate on the third day among days 1, 3 and 5. Doses of 10 3.0 to 10 4.7 TCID 50 /ml were administered to juvenile olive flounder across three farms. Results showed resistance to infection below 10 3.4 TCID 50 /ml at 15 °C. While infection frequency varied by concentration, higher concentrations correlated with more infections. Nonetheless, viral copy numbers did not differ significantly among infected fish at varying concentrations. This study underscores the need for early VHSV management and contributes essential data for pathogenicity assessment and foundational knowledge.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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