Necrosensor: a genetically encoded fluorescent sensor for visualizing necrosis in Drosophila.

Autor:	Nishida H; Division of Cell Physiology, Graduate School of Medicine, Kobe University, Kobe, 650-0017, Japan.; Physiological Genetics Laboratory, RIKEN CPR, Kobe, 650-0047, Japan., Albero AB; Laboratory for Epithelial Morphogenesis, RIKEN BDR, Kobe, 650-0047, Japan., Onoue K; Laboratory for Ultrastructural Research, RIKEN BDR, Kobe, 650-0047, Japan., Ikegawa Y; Laboratory of Molecular Cell Biology and Development, Kyoto University, Kobe, 650-0047, Japan.; Laboratory for Homeodynamics, RIKEN BDR, Kobe, 650-0047, Japan., Sulekh S; Laboratory for Homeodynamics, RIKEN BDR, Kobe, 650-0047, Japan.; Division of Developmental Biology and Regenerative Medicine, Graduate School of Medicine, Kobe University, Kobe, 650-0047, Japan., Sakizli U; Laboratory for Homeodynamics, RIKEN BDR, Kobe, 650-0047, Japan.; Division of Developmental Biology and Regenerative Medicine, Graduate School of Medicine, Kobe University, Kobe, 650-0047, Japan., Minami Y; Division of Cell Physiology, Graduate School of Medicine, Kobe University, Kobe, 650-0017, Japan., Yonemura S; Laboratory for Ultrastructural Research, RIKEN BDR, Kobe, 650-0047, Japan.; Department of Cell Biology, Tokushima University Graduate School of Medicine, Tokushima, 770-8503, Japan., Wang YC; Laboratory for Epithelial Morphogenesis, RIKEN BDR, Kobe, 650-0047, Japan., Yoo SK; Physiological Genetics Laboratory, RIKEN CPR, Kobe, 650-0047, Japan.; Laboratory for Homeodynamics, RIKEN BDR, Kobe, 650-0047, Japan.; Division of Developmental Biology and Regenerative Medicine, Graduate School of Medicine, Kobe University, Kobe, 650-0047, Japan.
Jazyk:	angličtina
Zdroj:	Biology open [Biol Open] 2024 Jan 15; Vol. 13 (1). Date of Electronic Publication: 2024 Jan 22.
DOI:	10.1242/bio.060104
Abstrakt:	Historically, necrosis has been considered a passive process, which is induced by extreme stress or damage. However, recent findings of necroptosis, a programmed form of necrosis, shed a new light on necrosis. It has been challenging to detect necrosis reliably in vivo, partly due to the lack of genetically encoded sensors to detect necrosis. This is in stark contrast with the availability of many genetically encoded biosensors for apoptosis. Here we developed Necrosensor, a genetically encoded fluorescent sensor that detects necrosis in Drosophila, by utilizing HMGB1, which is released from the nucleus as a damage-associated molecular pattern (DAMP). We demonstrate that Necrosensor is able to detect necrosis induced by various stresses in multiple tissues in both live and fixed conditions. Necrosensor also detects physiological necrosis that occurs during spermatogenesis in the testis. Using Necrosensor, we discovered previously unidentified, physiological necrosis of hemocyte progenitors in the hematopoietic lymph gland of developing larvae. This work provides a new transgenic system that enables in vivo detection of necrosis in real time without any intervention. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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