Antifungal susceptibility profile and biofilm-producing capability of Candida tropicalis isolates in a tertiary medical centre.

Autor:	Navarathinam SD; Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Medical Microbiology and Immunology, Kuala Lumpur, Malaysia., Neoh HM; UKM Medical Molecular Biology Institute (UMBI), Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia., Tan TL; Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Emergency Medicine, Kuala Lumpur, Malaysia., Wahab AA; Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Medical Microbiology and Immunology, Kuala Lumpur, Malaysia., Mohd Nizam Tzar MN; Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Medical Microbiology and Immunology, Kuala Lumpur, Malaysia., Ding CH; Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Medical Microbiology and Immunology, Kuala Lumpur, Malaysia. dingch@ppukm.ukm.edu.my.
Jazyk:	angličtina
Zdroj:	The Malaysian journal of pathology [Malays J Pathol] 2023 Dec; Vol. 45 (3), pp. 417-424.
Abstrakt:	Background: Candida tropicalis is a globally distributed yeast that has been popping up in the medical literature lately, albeit for unenviable reasons. C. tropicalis is associated with substantial morbidity, mortality as well as drug resistance. The aims of this study were to ascertain the antifungal susceptibility profile and the biofilm-producing capability of this notorious yeast in our centre. Methods: C. tropicalis isolates from sterile specimens were collected over a 12-month period. Conclusive identification was achieved biochemically with the ID 32 C kit. Susceptibility to nine antifungal agents was carried out using the colourimetric broth microdilution kit Sensititre YeastOne YO10. Biofilm-producing capability was evaluated by quantifying biomass formation spectrophotometrically following staining with crystal violet. Results: Twenty-four non-repetitive isolates of C. tropicalis were collected. The resistance rates to the triazole agents were 29.2% for fluconazole, 16.7% for itraconazole, 20.8% for voriconazole and 8.3% for posaconazole-the pan-azole resistance rate was identical to that of posaconazole. No resistance was recorded for amphotericin B, flucysosine or any of the echinocandins tested. A total of 16/24 (66.7%) isolates were categorized as high biomass producers and 8/24 (33.3%) were moderate biomass producers. None of our isolates were low biomass producers. Conclusion: The C. tropicalis isolates from our centre were resistant only to triazole agents, with the highest resistance rate being recorded for fluconazole and the lowest for posaconazole. While this is not by itself alarming, the fact that our isolates were prolific biofilm producers means that even azole-susceptible isolates can be paradoxically refractory to antifungal therapy.
Databáze:	MEDLINE
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