Subclinical circulation of Chicken infectious anaemia virus- A Sero-molecular study.

Autor: Sreekala SM; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India, 141004. drsree02vet@gmail.com.; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary & Animal Sciences University, Rampura Phul, Punjab, India, 151103. drsree02vet@gmail.com., Kaur G; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India, 141004., Dwivedi PN; Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana, Punjab, India, 141004.; Department of Veterinary Microbiology, Khalsa College of Veterinary and Animal Science, Amritsar, Punjab, India, 143002.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Mar; Vol. 55 (1), pp. 925-932. Date of Electronic Publication: 2023 Dec 29.
DOI: 10.1007/s42770-023-01223-8
Abstrakt: Chicken infectious anaemia-an important immunosuppressive viral disease of chicken-gained much attention in the recent past. Based on huge mortality and production loss observed in the fast-growing poultry sector, the present study aimed to find out the current status of the chicken infectious anaemia virus (CIAV), among chicken flocks in the Punjab state of India by sero-molecular study. The sera from the blood samples were tested for anti-CIAV antibodies by indirect ELISA and also compared with haematological parameters. DNA from sero-positive samples underwent PCR amplification, sequencing and phylogenetic analysis of the most conserved genomic region (VP3 gene) to detect viraemia in asymptomatic birds. The serological study using indirect ELISA showed a high sero-positivity of 77.27% in chicken flocks. Additionally, the present study also revealed the high molecular evidence (72.54%) of CIAV in apparently healthy birds. Genetic analysis showed that all CIAVs have conserved VP3 genes without any nucleotide substitutions, indicating presence of CIAV and its subclinical circulation among apparently healthy flocks. The wide distribution of CIAV among birds may be the reason for huge mortality and production loss. Further, it is suggested that studies be conducted to find out the co-involvement of CIAV with other immunosuppressive microbial agents and the immunosuppressive effect of CIAV in apparently healthy birds. Also, its role in vaccine failure and outbreaks of various other avian diseases needs to be explored.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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