Identification of CD38, CD97, and CD278 on the HIV surface using a novel flow virometry screening assay.

Autor: Burnie J; Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada.; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada., Fernandes C; Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada.; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada., Chaphekar D; Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada.; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada., Wei D; Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Ahmed S; Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada., Persaud AT; Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada.; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada., Khader N; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada., Cicala C; Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Arthos J; Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA., Tang VA; Flow Cytometry and Virometry Core Facility, Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, Canada., Guzzo C; Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada. christina.guzzo@utoronto.ca.; Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, ON, Canada. christina.guzzo@utoronto.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Dec 27; Vol. 13 (1), pp. 23025. Date of Electronic Publication: 2023 Dec 27.
DOI: 10.1038/s41598-023-50365-0
Abstrakt: While numerous cellular proteins in the HIV envelope are known to alter virus infection, methodology to rapidly phenotype the virion surface in a high throughput, single virion manner is lacking. Thus, many human proteins may exist on the virion surface that remain undescribed. Herein, we developed a novel flow virometry screening assay to discover new proteins on the surface of HIV particles. By screening a CD4 + T cell line and its progeny virions, along with four HIV isolates produced in primary cells, we discovered 59 new candidate proteins in the HIV envelope that were consistently detected across diverse HIV isolates. Among these discoveries, CD38, CD97, and CD278 were consistently present at high levels on virions when using orthogonal techniques to corroborate flow virometry results. This study yields new discoveries about virus biology and demonstrates the utility and feasibility of a novel flow virometry assay to phenotype individual virions.
(© 2023. The Author(s).)
Databáze: MEDLINE
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