Pathogenicity assessment of a bovine viral diarrhea virus type 1l (BVDV-1l) strain in experimentally infected calves.

Autor: Yeşilbağ K; Department of Virology, Bursa Uludag University, Faculty of Veterinary Medicine, 16059 Bursa, Turkiye. Electronic address: kyesilbag@uludag.edu.tr., Aytoğu G; Department of Virology, Bursa Uludag University, Faculty of Veterinary Medicine, 16059 Bursa, Turkiye., Kadiroğlu B; Department of Virology, Dicle University, Faculty of Veterinary Medicine, 21200 Diyarbakır, Turkiye., Ateş Ö; Department of Virology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03204 Afyonkarahisar, Turkiye., Toker EB; Department of Virology, Bursa Uludag University, Faculty of Veterinary Medicine, 16059 Bursa, Turkiye., Yaşar M; Department of Virology, Bursa Uludag University, Faculty of Veterinary Medicine, 16059 Bursa, Turkiye.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Feb; Vol. 289, pp. 109945. Date of Electronic Publication: 2023 Dec 15.
DOI: 10.1016/j.vetmic.2023.109945
Abstrakt: Bovine viral diarrhea is a widespread and economically important viral disease for livestock which can cause clinically diverse manifestations. The number of established BVDV subgenotypes has increased, not only the serological relationships of recently described subgenotypes but virulence and pathogenic characteristics have not yet been mostly elaborated. The dominant BVDV subgenotype in Turkiye was elaborated to be BVDV-1l, that involves more than half of field strains and there is no scientific data to identify the pathogenicity of this strain so far. This study investigated the pathogenicity of a selected field strain (TR-72) from subgenotype BVDV-1l. Experimental infection was implemented by intranasal inoculation of the strain TR-72 (10 ×10 5.5 ) to four young calves which were previously not vaccinated and were free both for BVDV antibodies and antigens. Clinical changes as well as blood parameters, body temperature, and viremia were monitored for 14 days. Only mild clinical signs associated with respiratory signs of BVDV infection were observed. Detected clinical signs included nasal discharge, conjunctivitis, cough, fatigue, high rectal temperature reaching 40.7 ℃, and white blood cell counts depression started from the 2nd day and 40.4% decreased between the 12th and 14th days post-infection (poi). The presence of viremia was investigated by virus isolation, RT-PCR, and real-time RT-PCR from blood samples. The efficiency of experimental infection was established not only by observed clinical signs but also by virus isolation from blood leukocytes between the 5th and 8th days poi., virus detection was obtained by real-time PCR between the 3rd - 13th days poi. Besides, the recorded mild clinical signs, high fever, long duration of viremia , and high decrease in blood parameters obtained in this study, it was shown that the noncytopathogenic BVDV-1l strain TR-72 has a moderate virulence in naïve cattle.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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