Altered apoptosis and proliferation in milk cells and PBMc from BLV-infected bovines with different proviral loads: Possible role of the BCL-2 family proteins, TNF-alpha, and receptors.

Autor: Ladera Gómez ME; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET/UNCPBA/CICPBA), Facultad de Ciencias Veterinarias, UNCPBA, Paraje Arroyo Seco s/n, 7000 Tandil, Argentina., Nieto Farias MV; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET/UNCPBA/CICPBA), Facultad de Ciencias Veterinarias, UNCPBA, Paraje Arroyo Seco s/n, 7000 Tandil, Argentina., Rodríguez M; Área de Bioestadística, Facultad de Ciencias Veterinarias, UNCPBA, Paraje Arroyo Seco s/n, 7000 Tandil, Argentina., Váter A; Escuela de Educación Secundaria Agraria Nº1 'Dr. Ramón Santamarina', Tandil, Argentina., Ceriani MC; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET/UNCPBA/CICPBA), Facultad de Ciencias Veterinarias, UNCPBA, Paraje Arroyo Seco s/n, 7000 Tandil, Argentina., Dolcini GL; Laboratorio de Virología, Centro de Investigación Veterinaria de Tandil (CIVETAN, CONICET/UNCPBA/CICPBA), Facultad de Ciencias Veterinarias, UNCPBA, Paraje Arroyo Seco s/n, 7000 Tandil, Argentina. Electronic address: gdolcini@vet.unicen.edu.ar.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2024 Feb; Vol. 268, pp. 110703. Date of Electronic Publication: 2023 Dec 14.
DOI: 10.1016/j.vetimm.2023.110703
Abstrakt: Bovines infected by bovine leukemia virus (BLV) are characterized by presenting low proviral load (LPL) or high proviral load (HPL). It is reported that animals with HPL in peripheral blood mononuclear cells (PBMCs) present a decrease in apoptosis, an increase in viability and the proliferation rate, while animals that maintain an LPL have an intrinsic ability to control the infection, presenting an increased apoptosis rate of their PBMCs. However, there is little information on the effect of BLV on these mechanisms when the virus infects somatic milk cells (SC). This study investigates the mechanisms underlying apoptosis in milk and blood from BLV-infected animals with HPL and LPL. Relative levels of mRNA of tumor necrosis factor-α (TNF-α), TNF receptor 1 (TNF-RI), TNF receptor 2 (TNF-RII), anti-apoptotic B-cell lymphoma 2 protein (Bcl-2), and pro-apoptotic Bcl-2-like protein 4 (Bax) were measured in SC and PBMCs using quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay. A significant decrease in the expression of TNF-α in SC from HPL animals vs non-infected bovines was observed, but the infection in SC with BLV did not show a modulation on the expression of TNF receptors. A significant increase in TNF-RI expression in PBMCs from HPL bovines compared to LPL bovines was observed. No significant differences in PBMCs between HPL and LPL compared to non-infected animals concerning TNF-α, TNF-RI, and TNF-RII expression were found. There was a significant increase of both Bcl-2 and Bax in SC from LPL compared to non-infected bovines, but the Bcl-2/Bax ratio showed an anti-apoptotic profile in LPL and HPL bovines compared to non-infected ones. Reduced mRNA expression levels of Bax were determined in the PBMCs from HPL compared to LPL subjects. In contrast, BLV-infected bovines did not differ significantly in the mRNA expression of Bax compared to non-infected bovines. Our data suggest that the increased mRNA expression of Bax corresponds to the late lactation state of bovine evaluated and the exacerbated increase of mRNA expression of Bcl-2 may be one of the mechanisms for the negative apoptosis regulation in the mammary gland induced by BLV infection. These results provide new insights into the mechanism of mammary cell death in HPL and LPL BLV-infected bovine mammary gland cells during lactation.
Competing Interests: Declaration of Competing Interest The authors declared no potential conflicts of interest concerning the research, authorship, publication of this article, and/or financial and personal relationships that could inappropriately influence this work.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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