Enhanced enrichment of collected airborne coronavirus and influenza virus samples via a ConA-coated microfluidic chip for PCR detection.

Autor: Piri A; Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea., Hyun KA; Korea Electronics Technology Institute (KETI), Seongnam-si, Gyeonggi-do 13509, Republic of Korea., Jung HI; Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: uridle7@yonsei.ac.kr., Nam KS; Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea., Hwang J; Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: hwangjh@yonsei.ac.kr.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Mar 05; Vol. 465, pp. 133249. Date of Electronic Publication: 2023 Dec 14.
DOI: 10.1016/j.jhazmat.2023.133249
Abstrakt: The severe acute respiratory syndrome (SARS-CoV-2) outbreak triggered global concern and emphasized the importance of virus monitoring. During a seasonal influenza A outbreak, relatively low concentrations of 10 3 -10 4 viral genome copies are available per 1 m 3 of air, which makes detection and monitoring very challenging because the limit of detection of most polymerase chain reaction (PCR) devices is approximately 10 3 viral genome copies/mL. In response to the urgent need for the rapid detection of airborne coronaviruses and influenza viruses, an electrostatic aerosol-to-hydrosol (ATH) sampler was combined with a concanavalin A (ConA)-coated high-throughput microfluidic chip. The samples were then used for PCR detection. The results revealed that the enrichment capacity of the ATH sampler was 30,000-fold for both HCoV-229E and H1N1 influenza virus, whereas the enrichment capacities provided by the ConA-coated microfluidic chip were 8-fold and 16-fold for HCoV-229E and H1N1 virus, respectively. Thus, the total enrichment capacities of our combined ATH sampler and ConA-coated microfluidic chip were 2.4 × 10 5 -fold and 4.8 × 10 5 -fold for HCoV-229E and H1N1 virus, respectively. This methodology significantly improves PCR detection by providing a higher concentration of viable samples.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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