Chelation chemistry of manganese-52 for PET imaging applications.

Autor: Omweri JM; Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL 35205, USA; Department of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA., Tekin V; Department of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA., Saini S; Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL 35205, USA; Department of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA., Houson HA; Department of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA., Jayawardana SB; Department of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL 35487, USA., Decato DA; Department of Chemistry and Biochemistry, University of Montana, MT 59812, USA., Wijeratne GB; Department of Chemistry and Biochemistry, University of Alabama, Tuscaloosa, AL 35487, USA., Lapi SE; Department of Chemistry, University of Alabama at Birmingham, Birmingham, AL 35205, USA; Department of Radiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. Electronic address: lapi@uab.edu.
Jazyk: angličtina
Zdroj: Nuclear medicine and biology [Nucl Med Biol] 2024 Jan-Feb; Vol. 128-129, pp. 108874. Date of Electronic Publication: 2023 Dec 23.
DOI: 10.1016/j.nucmedbio.2023.108874
Abstrakt: Introduction: Due to its decay and chemical properties, interest in manganese-52 has increased for development of long-lived PET radiopharmaceuticals. Its long half-life of 5.6 days, low average positron energy (242 keV), and sufficient positron decay branching ratio make it suitable for radiolabeling macromolecules for investigating slow biological processes. This work aims to establish suitable chelators for manganese-52 that can be radiolabeled at mild conditions through the evaluation of commercially available chelators.
Methods: Manganese-52 was produced through the nuclear reaction Nat Cr(p,n) 52 Mn by irradiation of natural chromium targets on a TR24 cyclotron followed by purification through ion exchange chromatography. The radiolabeling efficiencies of chelators: DOTA, DiAmsar, TETA, DO3A, NOTA, 4'-Formylbenzo-15-crown-5, Oxo-DO3A, and DFO, were assessed by investigating the impact of pH, buffer type, and temperature. In vitro stability of [ 52 Mn]Mn(DO3A) - , [ 52 Mn]Mn(Oxo-DO3A) - , and [ 52 Mn]Mn(DOTA) 2- were evaluated in mouse serum. The radiocomplexes were also evaluated in vivo in mice. Crystals of [Mn(Oxo-DO3A)] - were synthesized by reacting Oxo-DO3A with MnCl 2 and characterized by single crystal X-ray diffraction.
Results: Yields of 185 ± 19 MBq (5.0 ± 0.5 mCi) (n = 4) of manganese-52 were produced at the end of a 4 h, 15 μA, bombardment with 12.5 MeV protons. NOTA, DO3A, DOTA, and Oxo-DO3A chelators were readily radiolabeled with >96 % radiochemical purity at all conditions. Manganese radiocomplexes of Oxo-DO3A, DOTA, and DO3A remained stable in vitro up to 5 days and exhibited different biodistribution profiles compared to [ 52 Mn]MnCl 2 . The solid-state structure of Mn-Oxo-DO3A complex was determined by single-crystal X-ray diffraction.
Conclusions: DO3A and Oxo-DO3A are suitable chelators for manganese-52 which are readily radiolabeled at mild conditions with high molar activity, and demonstrate both in vitro and in vivo stability.
Competing Interests: Declaration of competing interest The authors declare no known competing interests.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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