Modulation of intestinal TLR4 expression in infants with neonatal opioid withdrawal syndrome.

Autor: Barnett RC; Department of Pediatrics, Marshall University, Joan C. Edwards School of Medicine, Huntington, WV, USA., Lewis AN; Division of Neonatal-Perinatal Medicine, Department of Pediatrics, Saint Louis University, Cardinal Glennon Children's Hospital, St. Louis, MO, USA., Gong Q; Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO, USA., Preston DL; Department of Pediatrics, Marshall University, Joan C. Edwards School of Medicine, Huntington, WV, USA., Frazer LC; Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO, USA.; Division of Neonatal-Perinatal Medicine, Department of Pediatrics, University of North Carolina Children's Hospital, Chapel Hill, NC, USA., Werthammer JW; Department of Pediatrics, Marshall University, Joan C. Edwards School of Medicine, Huntington, WV, USA., Good M; Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO, USA. mistygood@unc.edu.; Division of Neonatal-Perinatal Medicine, Department of Pediatrics, University of North Carolina Children's Hospital, Chapel Hill, NC, USA. mistygood@unc.edu.
Jazyk: angličtina
Zdroj: Journal of perinatology : official journal of the California Perinatal Association [J Perinatol] 2024 Aug; Vol. 44 (8), pp. 1125-1131. Date of Electronic Publication: 2023 Dec 27.
DOI: 10.1038/s41372-023-01859-2
Abstrakt: Objective: Neonatal Opioid Withdrawal Syndrome (NOWS) has been associated with the development of necrotizing enterocolitis (NEC) in term and late-preterm neonates. In this study, we used stool gene expression to determine if an increase in baseline inflammation in the intestine of infants with NOWS is associated with these findings.
Study Design: Stool samples were prospectively collected between days 1-3 and days 4-9 after delivery for opioid-exposed ( n = 9) or non-exposed neonates (n = 8). Stool gene expression for TLR4 and HMGB1 was determined via real-time PCR.
Results: TLR4 expression was higher in the stool of the non-exposed group in both time periods, between days 1-3 (P < 0.0001) and days 4-9 (P < 0.05) after delivery. No significant difference in HMGB1 expression was found at either time point (P > 0.05).
Conclusion: These findings point to an important interplay between opioid exposure and/or NOWS and the inflammatory milieu of the neonatal intestine.
(© 2023. The Author(s).)
Databáze: MEDLINE
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