Novel bioanalytical strategy using isotope pattern deconvolution and ICP-QMS for the study of iron incorporation in erythrocytes: An insight to better assessment.

Autor: Braz BF; Universidade Federal do Rio de Janeiro, Departamento de Química Analítica, Avenida Athos da Silveira Ramos 149, CT, Bloco A, 5° andar, Rio de Janeiro, RJ, 21941-909, Brazil., Omena J; Universidade do Estado do Rio de Janeiro, Instituto de Nutrição, Rio de Janeiro, Brazil., Voll VM; Universidade do Estado do Rio de Janeiro, Instituto de Nutrição, Rio de Janeiro, Brazil., Citelli M; Universidade do Estado do Rio de Janeiro, Instituto de Nutrição, Rio de Janeiro, Brazil., Rodrigues CDSC; Universidade do Estado do Rio de Janeiro, Instituto de Nutrição, Rio de Janeiro, Brazil., Cincotto FH; Universidade Federal do Rio de Janeiro, Departamento de Química Analítica, Avenida Athos da Silveira Ramos 149, CT, Bloco A, 5° andar, Rio de Janeiro, RJ, 21941-909, Brazil; National Institute of Science and Technology in Bioanalytics, Campinas, SP, Brazil., Fernández-Sánchez ML; University of Oviedo, Physical and Analytical Chemistry Department, Oviedo, Spain., Santelli RE; Universidade Federal do Rio de Janeiro, Departamento de Química Analítica, Avenida Athos da Silveira Ramos 149, CT, Bloco A, 5° andar, Rio de Janeiro, RJ, 21941-909, Brazil; National Institute of Science and Technology in Bioanalytics, Campinas, SP, Brazil. Electronic address: santelli@iq.ufrj.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Apr 01; Vol. 270, pp. 125579. Date of Electronic Publication: 2023 Dec 20.
DOI: 10.1016/j.talanta.2023.125579
Abstrakt: Iron is an essential element for human life and its nutritional status in the human body is directly linked to human health. More than 1015 atoms of iron per second are necessary for the maintenance of haemoglobin formation. To predict iron bioavailability three approaches are normally employed: (a) faecal recovery; (b) plasma appearance; and (c) erythrocyte incorporation (the most used). Isotope Pattern Deconvolution (IPD) is a mathematical tool that allows the isolation of distinct isotope signatures from mixtures of natural abundance and enriched tracers. In this work we propose a novel strategy to assess erythrocyte iron incorporation, based on the use of an iron stable isotope ( 57 Fe) and the IPD concept. This strategy allows direct calculation of the exogenous concentration of 57 Fe incorporated into RBCs after supplementation. In this way, to determine the mass of iron incorporated into erythrocytes, the unique prediction that must be made is the blood volume, estimate to reproduce the natural dilution of the tracer ( 57 Fe) in the blood. This novel bioanalytical approach was applied for the measurements of iron incorporation and further iron absorption studies in humans, using a group of twelve healthy participants, that should be further evaluated for the assessment of other chemical elements that could be of health concerns and directly impact society.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ricardo Erthal Santelli reports financial support was provided by National Council for Scientific and Technological Development. Ricardo Erthal Santelli reports financial support was provided by Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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