Transcriptomic and proteomic analyses of Mycobacterium tuberculosis strains isolated from tuberculous meningitis patients.

Autor:	Krishnakumariamma K; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India., Ellappan K; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India., Kadhiravan T; Department of Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India., Alex A; CIIMAR/CIMAR, Interdisciplinary Centre of Marine and Environmental Research, Matosinhos; Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal., Kumar SV; Department of Pulmonary Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India., Muthaiah M; Department of Microbiology, Intermediate Reference Laboratory, Government Hospital for Chest Diseases, Puducherry, India., Joseph NM; Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India.
Jazyk:	angličtina
Zdroj:	International journal of mycobacteriology [Int J Mycobacteriol] 2023 Oct-Dec; Vol. 12 (4), pp. 420-428.
DOI:	10.4103/ijmy.ijmy_159_23
Abstrakt:	Background: Tuberculous meningitis (TBM) is caused by the dissemination of Mycobacterium tuberculosis (MTB) from the primary site of infection to the central nervous system. However, the bacterial factors associated with the pathogenesis of TBM remain unclear. This study employed transcriptomic and proteomic methods to comprehensively analyze the changes in genes and proteins and their associated pathways in MTB strains isolated from cerebrospinal fluid (CSF) of TBM and sputum of pulmonary TB (PTB) cases. Methodology: Five MTB strains were subjected to OMICs (transcriptomic and proteomic) analysis. Among five MTB strains, two were isolated from CSF and sputum samples of the same patient with PTB and TBM infections, one from the sputum of a different PTB patient, and a strain obtained from the CSF of another TBM patient. H37Rv was used as a reference strain. The reliability of transcriptomic results was validated by real time polymerase chain reaction with selected genes from 100 MTB isolates (CSF, 50 and sputum, 50). Results: The transcriptomic study revealed that overlapping differentially expressed genes of MTB strains isolated from TBM patients showed featured enrichment in benzoate degradation, lysine degradation, tryptophan metabolism, fatty acid degradation, ATP binding cassette transporters, microbial metabolism in diverse environments, biosynthesis of antibiotics, and metabolic pathways. Eleven genes were upregulated, and four were downregulated in MTB strains isolated from TBM compared to PTB. From proteomic analysis, we identified three candidate proteins belonging to plasminogen binding proteins (PBP) (enolase, dnaK, and isocitrate lyase 1) that were significantly upregulated in MTB strains isolated from TBM. Conclusion: Overall, the transcriptomic and proteomic analyses provided an important base for understanding the unique feature of TBM pathogenesis. To the best of our knowledge, this is the first report highlighting the importance of PBPs on TBM pathogenesis. Competing Interests: None
Databáze:	MEDLINE
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