The Effect of Hydroalcoholic Extract of Quercus brantii and Artemisia aucheri Boiss Against Trichomonas vaginalis In vitro .
| Autor: | Rahdar M; Ahvaz Jundishapur University of Medical Sciences, Department of Parasitology, Ahvaz, Iran., Ramezaninia ST; Isfahan University Faculty of Medicine, Department of Parasitology, Isfahan, Iran., Karami MF; Ahvaz Jundishapur University of Medical Sciences, Department of Parasitology, Ahvaz, Iran., Hasanzadeh A; Ahvaz Jundishapur University Faculty of Medical Sciences, Student Research Committee, Ahvaz, Iran., Yousefi HA; Isfahan University Faculty of Medicine, Department of Parasitology, Isfahan, Iran., Zargar A; Andra University, Student Sri Venkateswara College of Pharmacy, India. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Turkiye parazitolojii dergisi [Turkiye Parazitol Derg] 2023 Dec 27; Vol. 47 (4), pp. 214-219. |
| DOI: | 10.4274/tpd.galenos.2023.55264 |
| Abstrakt: | Objective: Trichomoniasis is the most common sexually transmitted protozoan infection worldwide. Metronidazole is widely considered as the drug of choice for treating of trichomoniasis but considering its potential side effects, we aimed to assess the therapeutic influences of hydro-alcoholic extracts of Quercus brantii and Artemisia aucheri Boiss as alternative medications against Trichomonas vaginalis (T. vaginalis) . Methods: The trophozoites were cultured in TYI-S-33 medium at a density of 5x10 5 trophozoites/mL. Subsequently, they were incubated with varying concentrations of the plant extracts (32, 64, 125, 250, 500, and 1,000 μg/mL) and metronidazole (16, 32, 64, 125, 250, and 500 μg/mL), as the positive control. The number of trophozoites in each well plate was quantified after 2, 4, 6, 24, 48, and 72 hours using trypan blue staining. Finally, the viability of the parasite was assessed by vital methylene blue staining. Results: The hydro-alcoholic extracts of Q. brantii and A. aucheri Boiss at concentrations of 125, 250, 500, and 1,000 μg/mL demonstrated significant efficacy against the parasite. Our findings indicated that the minimum effective concentrations were 125 μg/mL and hydro-alcoholic extracts of Q. brantii and A. aucheri Boiss have the ability to effectively eliminate T. vaginalis after 48 and 72 hours of treatment. Conclusion: The findings of the present study showed that hydro-alcoholic extract of Q. brantii and A. aucheri Boiss can induce death in T. vaginalis . However, further complementary in vivo studies are needed to assess the components of these plants in the treatment of T. vaginalis . |
| Databáze: | MEDLINE |
| Externí odkaz: |
|