Comparison of the efficacy of combined budesonide and fexofenadine versus combined fluticasone propionate and fexofenadine on the expression of class-4 semaphorins and their receptors in the peripheral blood cells of patients with allergic rhinitis.

Autor:	Asadi G; Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Feizollahi P; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Rajabinejad M; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.; Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran., Falahi S; Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Rezaei Varmaziar F; Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Faryadi E; Student Research Committee, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Gorgin Karaji A; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Salari F; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran., Rezaiemanesh A; Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Nov 28; Vol. 10 (1), pp. e22924. Date of Electronic Publication: 2023 Nov 28 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2023.e22924
Abstrakt:	Background: Allergic rhinitis (AR) is a common immunoglobulin (Ig) E-mediated disease. This study aimed to evaluate the gene expression levels of class 4 semaphorins and their receptors in AR patients before and after treatment with budesonide and fexofenadine (B/F) compared to fluticasone propionate and fexofenadine (FP/F). Methods: In this study, 29 AR patients (age 34.4 ± 1.2 years, 18 men and 11 women) were treated with B/F, and 24 AR patients (age 32.8 ± 1.9 years, 15 men and 9 women) were treated with FP/F for one month. Before and after treatment, peripheral blood samples were taken from patients. The expression levels of SEMA4A , SEMA4C , SEMA4D , Plexin-B2 , and Plexin-D1 genes were measured using the qPCR method. In addition, the serum levels of IgE were measured using an enzyme-linked immunosorbent assay (ELISA). Results: The expression levels of SEMA4A ( P  = 0.011), 4C ( P  = 0.017), Plexin-B2 ( P  = 0.0005), and Plexin-D1 ( P  = 0.008) remarkably increased in AR patients treated with B/F. Our results show a significant reduction in the gene expression levels of SEMA4A ( P  = 0.002), 4C ( P  = 0.014), 4D ( P  = 0.003), Plexin-B2 ( P  = 0.033), and Plexin-D1 ( P  = 0.035) after treatment with FP/F. The serum levels of IgE increased in FP/F treated group ( P  = 0.017) and conversely decreased in the treated group with B/F ( P  = 0.019). Moreover, the percentages of eosinophils were reduced in both FP/F and B/F groups ( P  = 0.015 and P  = 0.0001, respectively). Conclusion: In conclusion, concomitant use of fexofenadine and fluticasone propionate reduced SEMA4A , 4C, 4D, Plexin-B2 , and Plexin-D1 , while the SEMA4A , 4C, Plexin-B2 , and Plexin-D1 gene expression levels were increased in the patient group treated with B/F. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.)
Databáze:	MEDLINE
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