Fibroblast growth factor 8b (FGF-8b) enhances myogenesis and inhibits adipogenesis in rotator cuff muscle cell populations in vitro.

Autor:	Otsuka T; The Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Storrs, CT 06269.; Raymond and Beverly Sackler Center for Biomedical, Biological, Physical, and Engineering Sciences, University of Connecticut Health Center, Farmington, CT 06030., Kan HM; The Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Storrs, CT 06269.; Raymond and Beverly Sackler Center for Biomedical, Biological, Physical, and Engineering Sciences, University of Connecticut Health Center, Farmington, CT 06030., Mengsteab PY; The Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Storrs, CT 06269.; Raymond and Beverly Sackler Center for Biomedical, Biological, Physical, and Engineering Sciences, University of Connecticut Health Center, Farmington, CT 06030.; Department of Orthopedic Surgery, University of Connecticut Health Center, Farmington, CT 06030.; Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269., Tyson B; The Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Storrs, CT 06269., Laurencin CT; The Cato T. Laurencin Institute for Regenerative Engineering, University of Connecticut, Storrs, CT 06269.; Raymond and Beverly Sackler Center for Biomedical, Biological, Physical, and Engineering Sciences, University of Connecticut Health Center, Farmington, CT 06030.; Department of Orthopedic Surgery, University of Connecticut Health Center, Farmington, CT 06030.; Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269.; Department of Materials Science and Engineering, University of Connecticut, Storrs, CT 06269.; Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jan 02; Vol. 121 (1), pp. e2314585121. Date of Electronic Publication: 2023 Dec 26.
DOI:	10.1073/pnas.2314585121
Abstrakt:	Fatty expansion is one of the features of muscle degeneration due to muscle injuries, and its presence interferes with muscle regeneration. Specifically, poor clinical outcomes have been linked to fatty expansion in rotator cuff tears and repairs. Our group recently found that fibroblast growth factor 8b (FGF-8b) inhibits adipogenic differentiation and promotes myofiber formation of mesenchymal stem cells in vitro. This led us to hypothesize that FGF-8b could similarly control the fate of muscle-specific cell populations derived from rotator cuff muscle involved in muscle repair following rotator cuff injury. In this study, we isolate fibro-adipogenic progenitor cells (FAPs) and satellite stem cells (SCs) from rat rotator cuff muscle tissue and analyzed the effects of FGF-8b supplementation. Utilizing a cell plating protocol, we successfully isolate FAPs-rich fibroblasts (FIBs) and SCs-rich muscle progenitor cells (MPCs). Subsequently, we demonstrate that FIB adipogenic differentiation can be inhibited by FGF-8b, while MPC myogenic differentiation can be enhanced by FGF-8b. We further demonstrate that phosphorylated ERK due to FGF-8b leads to the inhibition of adipogenesis in FIBs and SCs maintenance and myofiber formation in MPCs. Together, these findings demonstrate the powerful potential of FGF-8b for rotator cuff repair by altering the fate of muscle undergoing degeneration. Competing Interests: Competing interests statement:The patent filings to disclose, entitled “Use of Fibroblast Growth Factor-8 for Tissue Regeneration” (Publication No.: WO/2022/150291).
Databáze:	MEDLINE
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