Differential Regulation of the STING Pathway in Human Papillomavirus-Positive and -Negative Head and Neck Cancers.

Autor:	Saulters EL; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, United Kingdom., Kennedy PT; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, United Kingdom., Carter RJ; Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool, United Kingdom., Alsufyani A; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, United Kingdom., Jones TM; Department of Molecular and Clinical Cancer Medicine, University of Liverpool, Liverpool, United Kingdom., Woolley JF; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, United Kingdom., Dahal LN; Department of Pharmacology and Therapeutics, University of Liverpool, Liverpool, United Kingdom.
Jazyk:	angličtina
Zdroj:	Cancer research communications [Cancer Res Commun] 2024 Jan 16; Vol. 4 (1), pp. 118-133.
DOI:	10.1158/2767-9764.CRC-23-0299
Abstrakt:	Squamous cell carcinomas, which arise from the cells that line the mucosal surfaces of the head and neck, represent the most common type of head and neck cancers (HNSCC). Human papillomavirus (HPV) infection has been strongly associated with the development of oropharyngeal cancers, which are cancers that occur in the back of the throat, including the tonsils and base of the tongue. HNSCCs with and without HPV infection have distinct pathology, with HPV-positive patients having higher levels of immune infiltration, activation in the tumor microenvironment and better response to radiation and chemotherapy. It is, however, unclear whether HPV infection in HNSCCs has the potential to activate innate-immune sensing pathways and if these cancers possess intrinsic immunogenicity associated with HPV infection. Here we investigate the innate immune stimulator of interferon genes (STING) pathway and immune responses to STING activation in HNSCCs and uncover fundamental differences in the regulation of this pathway in cell lines versus primary human clinical specimens. We show that while STING is differentially expressed in HPV-positive and -negative HNSCC cell lines, they exhibit a gross functional defect in signaling through this pathway. However, STING activation in immune cell populations generated immune signatures predicted to elicit useful tumoricidal mechanisms. In contrast, IHC analysis of human tissue microarrays revealed enhanced STING expression in HPV-related tumors and high intratumoral expression of STING correlated with increased survival. Significance: STING is an important innate immune sensor of cytosolic DNA, inducing essential antiviral and antitumoral responses. This research shows that STING expression is enhanced in HPV-positive HNSCC patient tissue, with high intratumoral STING expression correlating with increased survival. In addition, STING activation in immune cell populations augmented antitumoral effects against HNSCCs, suggesting patients may benefit from the use of STING agonists in combination with traditional therapies. (© 2024 The Authors; Published by the American Association for Cancer Research.)
Databáze:	MEDLINE
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