ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to Saccharomyces cerevisiae mannan.

Autor:	Krylov VB; Laboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.; Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Kuznetsov AN; Laboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Polyanskaya AV; Laboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Tsarapaev PV; Laboratory of Synthetic Glycovaccines, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia., Yashunsky DV; Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia., Kushlinskii NE; N. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia., Nifantiev NE; Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in molecular biosciences [Front Mol Biosci] 2023 Dec 07; Vol. 10, pp. 1296828. Date of Electronic Publication: 2023 Dec 07 (Print Publication: 2023).
DOI:	10.3389/fmolb.2023.1296828
Abstrakt:	Mannans are polysaccharide antigens expressed on the cell wall of different fungal species including Saccharomyces cerevisiae and Candida spp. These fungi are components of the normal intestinal microflora, and the presence of antibodies to fungal antigens is known to reflect the features of the patient's immune system. Thus, titers of IgG and IgA antibodies against Saccharomyces cerevisiae mannan (ASCA) are markers for clinical diagnostics of inflammatory bowel diseases. The complex organization and heterogeneity of cell-wall mannans may reduce the quality and reproducibility of ELISA results due to interference by different antigenic epitopes. In this research, we analyzed the levels of IgG antibodies in the sera of healthy donors and patients with colorectal cancer using an array of synthetic oligosaccharides related to distinct fragments of fungal mannan. This study aimed to establish the influence of oligosaccharide structure on their antigenicity. Variations in the structure of the previously established ASCA epitope (changing type of linkage, chain length, and the presence of branches) significantly modified the ability of ligands to bind to circulating antibodies in blood sera. The study showed that surface presentation density of the ligand critically affects the results of enzyme immunoassay. The transition from natural coating antigens to their corresponding synthetic mimetics with a defined structure opens new opportunities for improving existing ELISA test systems, as well as developing diagnostic kits with new properties. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2023 Krylov, Kuznetsov, Polyanskaya, Tsarapaev, Yashunsky, Kushlinskii and Nifantiev.)
Databáze:	MEDLINE
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