Moxibustion influences hippocampal microglia polarization via IL-33/ST2 pathway in Alzheimer's disease mice.

Autor:	Li HY; Acupuncture-moxibustion and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China. 1558494627@qq.com., Shen Y; Acupuncture-moxibustion and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China., Xie LS; College of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610075. xielushuang@cdutcm.edu.cn., Wu QF; Acupuncture-moxibustion and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China., Yu SG; Acupuncture-moxibustion and Tuina College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
Jazyk:	English; Chinese
Zdroj:	Zhen ci yan jiu = Acupuncture research [Zhen Ci Yan Jiu] 2023 Dec 25; Vol. 48 (12), pp. 1202-1208.
DOI:	10.13702/j.1000-0607.20220877
Abstrakt:	Objectives: To observe the effect of moxibustion on the polarization of microglia towards M2 direction in Alzheimer's disease (AD) mice through the interleukin-33 (IL-33)/growth stimulating gene 2 protein (ST2) signaling pathway. Methods: Five-month-old APP/PS1 male mice were randomly divided into model and moxibustion (Moxi) groups, and C57BL/6J mice of the same age were as the control group, with 9 mice in each group. In the Moxi group, moxibustion was applied at "Baihui" (GV20) and "Yongquan" (KI1) for 30 min, once a day, 5 days a week for 4 weeks. The spatial learning memory ability was observed by the Morris water maze test. The relative expressions of IL-33 and ST2 in hippocampus were detected by Western blot. The positive expression of amyloid-β (Aβ), phosphorylated Tau (p-Tau), IL-33/ionized calcium binding adapter molecule 1(Iba-1), ST2/Iba-1, arginase 1 (Arg1)/Iba-1 and indu-cible nitric oxide synthase (iNOS)/Iba-1 in hippocampal CA1 region were detected by immunofluorescence. Results: Compared with the control group, the escape latency of the mice in the model group was prolonged ( P <0.001, P <0.01), the number of times to enter the effective area and the percentage of target quadrant swimming time were reduced ( P <0.001), the positive expression of both Aβ and p-Tau, the positive expression of iNOS/Iba-1 in the hippocampal CA1 region was increased ( P <0.001), while the expression of IL-33 and ST2 protein in hippocampal tissue, the positive expression levels of IL-33/Iba-1, ST2/Iba-1 and Arg1/Iba-1 in hippocampal CA1 region were all decreased ( P <0.05, P <0.001). After treatment, compared with the model group, the escape latency of the mice in the moxibustion group was shortened ( P <0.001, P <0.01), the number of entries into the effective area and the percentage of target quadrant swimming time were increased ( P <0.001), the positive expression of Aβ and p-Tau in the hippocampal CA1 region, and the positive expression of iNOS/Iba-1 were decreased ( P <0.001), while the expression of IL-33 and ST2 protein in the hippocampal tissue, the positive expression of IL-33/Iba-1, ST2/Iba-1 and Arg1/Iba-1 in hippocampal CA1 region were all increased ( P <0.05, P <0.01, P <0.001). Conclusions: Moxibustion can improve the spatial learning and memory abilities, reduce the pathological deposition of Aβ and p-Tau in APP/PS1 mice, which may be related to its function in up-regulating the IL-33/ST2 signaling pathway to regulate the polarization of microglia towards M2 direction.
Databáze:	MEDLINE
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