Metabolomic study of the estrogenic and anti-osteoporotic potential of Erythrina bidwillii leaf.

Autor: AbouZeid EM; Department of Pharmacognosy, National Research Centre, Giza, Egypt., Hussein RA; Department of Pharmacognosy, National Research Centre, Giza, Egypt., Salama AA; Department of Pharmacology, National Research Centre, Giza, Egypt., Youssef FS; Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt., El-Ahmady SH; Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt., Ammar NM; Department of Pharmacognosy, National Research Centre, Giza, Egypt., Afifi AH; Department of Pharmacognosy, National Research Centre, Giza, Egypt.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2024 Mar; Vol. 38 (3), pp. e5810. Date of Electronic Publication: 2023 Dec 25.
DOI: 10.1002/bmc.5810
Abstrakt: Erythrina bidwillii Lindl., Leguminosae, constitutes a valuable crop for horticulture and medicine; however, it is rarely investigated. Menopause is a crucial transitional period in women's health. Women worldwide consider the use of phytoestrogens as a safe hormone replacement therapy to alleviate detrimental menopausal symptoms. Thus, the discovery of novel phytoestrogens is highly demanded. The present study aimed to investigate, for the first time, the metabolomic profile and the estrogenic potential of E. bidwillii Lindl. leaf. Ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry and gas chromatography-mass spectrometry metabolite profiling revealed the prevalence of alkaloids, flavonoids, isoflavonoids and fatty acids. Additionally, five erythrinan alkaloids, cristanine A (1), 8-oxoerythraline (2), (+)-erythrinine (3), (+)-erythraline (4) and 8-oxoerythrinine (5), along with the isoflavonoid genistin (6), were isolated. Erythrina bidwillii leaf extract exhibited significant in vivo estrogenic, anti-osteoporotic, anti-hyperlipidemic, hepatoprotective, and nephroprotective activities, utilizing ovariectomized rat model. Moreover, ethyl acetate and hexane fractions possessed significant in vitro estrogeic potential on MCF-7 cell lines. An in silico study of the isolated metabolites revealed that (+)-erythrinine (3) and 8-oxoerythrinine (5) exhibited the highest affinity for ERα and ERβ, respectively, modeling them as potential estrogenic lead metabolites. Therefore, E. bidwillii leaf could be employed as promising hormone replacement therapy for postmenopausal women after thorough clinical trials.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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