Risks of using mitoquinone during in vitro maturation and its potential protective effects against lipotoxicity-induced oocyte mitochondrial stress.

Autor: Marei WFA; Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium. waleed.marei@uantwerpen.be.; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. waleed.marei@uantwerpen.be., Mohey-Elsaeed O; Department of Histology and Cytology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.; Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium., Pintelon I; Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium., Leroy JLMR; Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium.
Jazyk: angličtina
Zdroj: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2024 Feb; Vol. 41 (2), pp. 371-383. Date of Electronic Publication: 2023 Dec 26.
DOI: 10.1007/s10815-023-02994-7
Abstrakt: Purpose: Oxidative stress and mitochondrial dysfunction play central roles in reduced oocyte quality and infertility in obese patients. Mitochondria-targeted treatments containing co-enzyme Q10 such as mitoquinone (MitoQ) can increase mitochondrial antioxidative capacity; however, their safety and efficiency when supplemented to oocytes under lipotoxic conditions have not been described.
Methods: We tested the effect of different concentrations of MitoQ or its cationic carrier (TPP) (0, 0.1, 0.5, 1.0 μM each) during bovine oocyte IVM. Then, we tested the protective capacity of MitoQ (0.1 μM) against palmitic acid (PA)-induced lipotoxicity and mitochondrial dysfunction in oocytes.
Results: Exposure to MitoQ, or TPP only, at 1 μM significantly (P<0.05) reduced oocyte mitochondrial inner membrane potential (JC-1 staining) and resulted in reduced cleavage and blastocyst rates compared with solvent control. Lower concentrations of MitoQ or TPP had no effects on embryo development under control (PA-free) conditions. As expected, PA increased the levels of MMP and ROS in oocytes (CellROX staining) and reduced cleavage and blastocyst rates compared with the controls (P<0.05). These negative effects were ameliorated by 0.1 μM MitoQ. In contrast, 0.1 μM TPP alone had no protective effects. MitoQ also normalized the expression of HSP10 and TFAM, and partially normalized HSP60 in the produced blastocysts, indicating at least a partial alleviation of PA-induced mitochondrial stress.
Conclusion: Oocyte exposure to MitoQ may disturb mitochondrial bioenergetic functions and developmental capacity due to a TPP-induced cationic overload. A fine-tuned concentration of MitoQ can protect against lipotoxicity-induced mitochondrial stress during IVM and restore developmental competence and embryo quality.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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