Restoration of metabolic functional metrics from label-free, two-photon human tissue images using multiscale deep-learning-based denoising algorithms.

Autor:	Vora N; Tufts University, Department of Biomedical Engineering, Medford, Massachusetts, United States., Polleys CM; Tufts University, Department of Biomedical Engineering, Medford, Massachusetts, United States., Sakellariou F; Anatolia College, Thessaloniki, Greece., Georgalis G; Tufts University, Data Intensive Studies Center, Medford, Massachusetts, United States., Thieu HT; Tufts University School of Medicine, Tufts Medical Center, Department of Obstetrics and Gynecology, Boston, Massachusetts, United States., Genega EM; Tufts University School of Medicine, Tufts Medical Center, Department of Pathology and Laboratory Medicine, Boston, Massachusetts, United States., Jahanseir N; Tufts University School of Medicine, Tufts Medical Center, Department of Pathology and Laboratory Medicine, Boston, Massachusetts, United States., Patra A; Tufts University, Data Intensive Studies Center, Medford, Massachusetts, United States.; Tufts University, Department of Mathematics, Medford, Massachusetts, United States., Miller E; Tufts University, Department of Electrical and Computer Engineering, Medford, Massachusetts, United States.; Tufts University, Tufts Institute for Artificial Intelligence, Medford, Massachusetts, United States., Georgakoudi I; Tufts University, Department of Biomedical Engineering, Medford, Massachusetts, United States.
Jazyk:	angličtina
Zdroj:	Journal of biomedical optics [J Biomed Opt] 2023 Dec; Vol. 28 (12), pp. 126006. Date of Electronic Publication: 2023 Dec 22.
DOI:	10.1117/1.JBO.28.12.126006
Abstrakt:	Significance: Label-free, two-photon excited fluorescence (TPEF) imaging captures morphological and functional metabolic tissue changes and enables enhanced understanding of numerous diseases. However, noise and other artifacts present in these images severely complicate the extraction of biologically useful information. Aim: We aim to employ deep neural architectures in the synthesis of a multiscale denoising algorithm optimized for restoring metrics of metabolic activity from low-signal-to-noise ratio (SNR), TPEF images. Approach: TPEF images of reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavoproteins (FAD) from freshly excised human cervical tissues are used to assess the impact of various denoising models, preprocessing methods, and data on metrics of image quality and the recovery of six metrics of metabolic function from the images relative to ground truth images. Results: Optimized recovery of the redox ratio and mitochondrial organization is achieved using a novel algorithm based on deep denoising in the wavelet transform domain. This algorithm also leads to significant improvements in peak-SNR (PSNR) and structural similarity index measure (SSIM) for all images. Interestingly, other models yield even higher PSNR and SSIM improvements, but they are not optimal for recovery of metabolic function metrics. Conclusions: Denoising algorithms can recover diagnostically useful information from low SNR label-free TPEF images and will be useful for the clinical translation of such imaging. (© 2023 The Authors.)
Databáze:	MEDLINE
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