Impact of bacteriocin-producing strains on bacterial community composition in a simplified human intestinal microbiota.

Autor:	Ríos Colombo NS; APC Microbiome Ireland, University College Cork, Cork, Ireland., Perez-Ibarreche M; APC Microbiome Ireland, University College Cork, Cork, Ireland., Draper LA; APC Microbiome Ireland, University College Cork, Cork, Ireland., O'Connor PM; APC Microbiome Ireland, University College Cork, Cork, Ireland.; Teagasc Food Research Centre, Moorepark, Fermoy, Co., Cork, Ireland., Field D; APC Microbiome Ireland, University College Cork, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland., Ross RP; APC Microbiome Ireland, University College Cork, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland., Hill C; APC Microbiome Ireland, University College Cork, Cork, Ireland.; School of Microbiology, University College Cork, Cork, Ireland.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Dec 08; Vol. 14, pp. 1290697. Date of Electronic Publication: 2023 Dec 08 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1290697
Abstrakt:	Bacteriocins are antimicrobial peptides that have been studied for decades as food bio-preservatives or as alternatives to antibiotics. They also have potential as modulators of the gut microbiome, which has been linked to human health. However, it is difficult to predict a priori how bacteriocins will impact complex microbial communities through direct and indirect effects. Here we assess the effect of different bacteriocin-producing strains on a Simplified Human Intestinal Microbiota (SIHUMI) model, using a set of bacteriocin-producing strains (Bac+) and otherwise isogenic non-producers (Bac-). Bacteriocins from different classes and with different activity spectra were selected, including lantibiotics such as lacticin 3147 and nisin A, and pediocin-like bacteriocins such as pediocin PA-1 among other peptides. SIHUMI is a bacterial consortium of seven diverse human gut species that assembles to a predictable final composition in a particular growth medium. Each member can be individually tracked by qPCR. Bac+ and Bac- strains were superimposed on the SIHUMI system, and samples were taken at intervals up to 48 h. The genome copy number of each SIHUMI member was evaluated using specific primers. We establish that the composition of the community changes in response to the presence of either broad- or narrow-spectrum bacteriocin producers and confirm that there are significant off-target effects. These effects were analyzed considering antagonistic inter-species interactions within the SIHUMI community, providing a comprehensive insight into the possible mechanisms by which complex communities can be shaped by bacteriocins. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2023 Ríos Colombo, Perez-Ibarreche, Draper, O’Connor, Field, Ross and Hill.)
Databáze:	MEDLINE
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