Comparison of in vitro thyroxine (T4) metabolism between Wistar rat and human hepatocyte cultures.

Autor: Baze A; KaLy-Cell SAS, 20A rue du Général Leclerc, 67115 Plobsheim, France., Wiss L; KaLy-Cell SAS, 20A rue du Général Leclerc, 67115 Plobsheim, France., Horbal L; Pharmacelsus GmbH, Science Park 2, 66123 Saarbrüken, Germany., Biemel K; Pharmacelsus GmbH, Science Park 2, 66123 Saarbrüken, Germany., Asselin L; KaLy-Cell SAS, 20A rue du Général Leclerc, 67115 Plobsheim, France., Richert L; KaLy-Cell SAS, 20A rue du Général Leclerc, 67115 Plobsheim, France; Zylan SAS, 8 rue de la Haute Corniche, 67210 Obernai, France. Electronic address: zylan.lr@kaly-cell.com.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2024 Apr; Vol. 96, pp. 105763. Date of Electronic Publication: 2023 Dec 22.
DOI: 10.1016/j.tiv.2023.105763
Abstrakt: In vitro assays remain relatively new in exploring human relevance of liver, in particular nuclear receptor-mediated perturbations of the hypothalamus-pituitary-thyroid axis seen in rodents, mainly in the rat. Consistent with in vivo data, we confirm that thyroid hormone thyroxine metabolism was 9 times higher in primary rat hepatocytes (PRH) than in primary human hepatocytes (PHH) cultured in a 2D sandwich (2Dsw) configuration. In addition, thyroxine glucuronide (T4-G) was by far the major metabolite formed in both species (99.1% in PRH and 69.7% in PHH) followed by thyroxine sulfate (T4-S, 0.7% in PRH and 18.1% in PHH) and triiodothyronine/reverse triiodothyronine (T3/rT3, 0.2% in PRH and 12.2% in PHH). After a 7-day daily exposure to orphan receptor-mediated liver inducers, T4 metabolism was strongly increased in PRH, almost exclusively through increased T4-G formation. These results were consistent with the inductions of glucuronosyltransferase Ugt2b1 and canalicular transporter Mrp2. PHH also responded to activation of the three nuclear receptors, with mainly induction of glucuronosyltransferase UGT1A1 and canalicular transporter MRP2. Despite this, T4 disappearance rate and secreted T4 metabolites were only slightly increased in PHH. Overall, our data highlight that cryopreserved hepatocytes in 2Dsw culture allowing long-term exposure and species comparison are of major interest in improving liver-mediated human safety assessment.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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